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Science 4 March 1988:
Vol. 239. no. 4844, pp. 1137 - 1139
DOI: 10.1126/science.2830672
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Articles

Science, Vol 239, Issue 4844, 1137-1139
Copyright © 1988 by American Association for the Advancement of Science

articles

Estrogen-dependent in vitro transcription from the vitellogenin promoter in liver nuclear extracts

B Corthesy, R Hipskind, I Theulaz, and W Wahli

Institut de Biologie animale, Universite de Lausanne, Switzerland.

One approach to analyzing the molecular mechanisms of gene expression in vivo is to reconstitute these events in cell-free systems in vitro. Although there is some evidence for tissue-specific transcription in vitro, transcriptionally active extracts that mimic a steroid hormone-dependent enhancement of transcription have not been described. In the study reported here, nuclear extracts of liver from the frog Xenopus laevis were capable of estrogen-dependent induction of a homologous vitellogenin promoter that contained the estrogen-responsive element.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Functional Interactions between the Estrogen Receptor and the Transcription Activator Sp1 Regulate the Estrogen-dependent Transcriptional Activity of the Vitellogenin A1 io Promoter.
S. R. B. de Medeiros, G. Krey, A. K. Hihi, and W. Wahli (1997)
J. Biol. Chem. 272, 18250-18260
   Abstract »    Full Text »    PDF »
In vitro transcription enhancement by purified derivatives of the glucocorticoid receptor.
L. Freedman, S. Yoshinaga, J. Vanderbilt, and K. Yamamoto (1989)
Science 245, 298-301
   Abstract »    PDF »


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