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Science 29 January 1988:
Vol. 239. no. 4839, pp. 487 - 491
DOI: 10.1126/science.2448875
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Articles

Science, Vol 239, Issue 4839, 487-491
Copyright © 1988 by American Association for the Advancement of Science

articles

Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase

RK Saiki, DH Gelfand, S Stoffel, SJ Scharf, R Higuchi, GT Horn, KB Mullis, and HA Erlich

Cetus Corporation, Department of Human Genetics, Emeryville, CA 94608.

A thermostable DNA polymerase was used in an in vitro DNA amplification procedure, the polymerase chain reaction. The enzyme, isolated from Thermus aquaticus, greatly simplifies the procedure and, by enabling the amplification reaction to be performed at higher temperatures, significantly improves the specificity, yield, sensitivity, and length of products that can be amplified. Single-copy genomic sequences were amplified by a factor of more than 10 million with very high specificity, and DNA segments up to 2000 base pairs were readily amplified. In addition, the method was used to amplify and detect a target DNA molecule present only once in a sample of 10(5) cells.


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Cadmium Accumulation and DNA Homology with Metal Resistance Genes in Sulfate-Reducing Bacteria.
N. Naz, H. K. Young, N. Ahmed, and G. M. Gadd (2005)
Appl. Envir. Microbiol. 71, 4610-4618
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A phylogenetic reappraisal of the Peltophorum group (Caesalpinieae: Leguminosae) based on the chloroplast trnL-F, rbcL and rps16 sequence data.
E. M. Haston, G. P. Lewis, and J. A. Hawkins (2005)
Am. J. Botany 92, 1359-1371
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Parvovirus B19 infection associated with unilateral cervical lymphadenopathy, apoptotic sinus histiocytosis, and prolonged fatigue.
T Knosel, H Meisel, A Borgmann, T Riebel, V Krenn, C Schewe, and I Petersen (2005)
J. Clin. Pathol. 58, 872-875
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In vivo antitumor activity of the NF-{kappa}B inhibitor dehydroxymethylepoxyquinomicin in a mouse model of adult T-cell leukemia.
T. Ohsugi, R. Horie, T. Kumasaka, A. Ishida, T. Ishida, K. Yamaguchi, T. Watanabe, K. Umezawa, and T. Urano (2005)
Carcinogenesis 26, 1382-1388
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Basal Body Duplication and Maintenance Require One Member of the Tetrahymena thermophila Centrin Gene Family.
A. J. Stemm-Wolf, G. Morgan, T. H. Giddings Jr., E. A. White, R. Marchione, H. B. McDonald, and M. Winey (2005)
Mol. Biol. Cell 16, 3606-3619
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Novel Approach to Mapping of Resistance Mutations in Whole Genomes by Using Restriction Enzyme Modulation of Transformation Efficiency.
C. G. Lerner, S. J. Kakavas, C. Wagner, R. T. Chang, P. J. Merta, X. Ruan, R. E. Metzger, and B. A. Beutel (2005)
Antimicrob. Agents Chemother. 49, 2767-2777
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