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Science 18 December 1987:
Vol. 238. no. 4834, pp. 1722 - 1725
DOI: 10.1126/science.3686011
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Articles

Science, Vol 238, Issue 4834, 1722-1725
Copyright © 1987 by American Association for the Advancement of Science

articles

Structure of a psoralen cross-linked DNA in solution by nuclear magnetic resonance

MT Tomic, DE Wemmer, and SH Kim

Department of Chemistry, University of California, Berkeley 94720.

One- and two-dimensional nuclear magnetic resonance (NMR) methods were used to determine a three-dimensional model of an eight-base-pair DNA fragment (d-GGGTACCC) cross-linked with psoralen in solution. Two-dimensional nuclear Overhauser effect experiments were used to assign the spectrum and estimate distances for 171 proton pairs in the cross-linked DNA. The NMR-derived model shows a 53 degree bend into the major groove that occurs primarily at the site of drug addition and a 56 degree unwinding that spans the eight-base-pair duplex.


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Purification and Enzymic Properties of Mot1 ATPase, a Regulator of Basal Transcription in the Yeast Saccharomyces cerevisiae.
J. I. Adamkewicz, C. G. F. Mueller, K. E. Hansen, W. A. Prud'homme, and J. Thorner (2000)
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