Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Science 18 December 1987:
Vol. 238. no. 4834, pp. 1720 - 1722
DOI: 10.1126/science.3686010
Prev | Table of Contents | Next

Articles

Science, Vol 238, Issue 4834, 1720-1722
Copyright © 1987 by American Association for the Advancement of Science

articles

Sodium-calcium exchange in heart: membrane currents and changes in [Ca2+]i

L Barcenas-Ruiz, DJ Beuckelmann, and WG Wier

Department of Physiology, University of Maryland School of Medicine, Baltimore.

Recordings have been made of changes in intracellular calcium ion concentration ([Ca2+]i) that can be attributed to the operation of an electrogenic, voltage-dependent sodium-calcium (Na-Ca) exchanger in mammalian heart cells. Guinea pig ventricular myocytes under voltage clamp were perfused internally with fura-2, a fluorescent Ca2+-indicator, and changes in [Ca2+]i and membrane current that resulted from Na-Ca exchange were identified through the use of various organic channel blockers and impermeant ions. Depolarization of cells elicited slow increases in [Ca2+]i, with the maximum increase depending on internal [Na+], external [Ca2+], and membrane voltage. Repolarization was associated with net Ca2+ efflux and a decline in the inward current that developed instantaneously upon repolarization. The relation between [Ca2+]i and current was linear, and the slope was made steeper by hyperpolarization.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
The mechanism and significance of the slow changes of ventricular action potential duration following a change of heart rate.
D. A. Eisner, K. M. Dibb, and A. W. Trafford (2009)
Exp Physiol 94, 520-528
   Abstract »    Full Text »    PDF »
Modulation of Sarcoplasmic Reticulum Function by PST2744 [Istaroxime; (E,Z)-3-((2-Aminoethoxy)imino) Androstane-6,17-dione Hydrochloride)] in a Pressure-Overload Heart Failure Model.
M. Rocchetti, M. Alemanni, G. Mostacciuolo, P. Barassi, C. Altomare, R. Chisci, R. Micheletti, P. Ferrari, and A. Zaza (2008)
J. Pharmacol. Exp. Ther. 326, 957-965
   Abstract »    Full Text »    PDF »
Na+/Ca2+ exchange current (INa/Ca) and sarcoplasmic reticulum Ca2+ release in catecholamine-induced cardiac hypertrophy.
A. Chorvatova, G. Hart, and M. Hussain (2004)
Cardiovasc Res 61, 278-287
   Abstract »    Full Text »    PDF »
Influence of age and run training on cardiac Na+/Ca2+ exchange.
L. C. Mace, B. M. Palmer, D. A. Brown, K. N. Jew, J. M. Lynch, J. M. Glunt, T. A. Parsons, J. Y. Cheung, and R. L. Moore (2003)
J Appl Physiol 95, 1994-2003
   Abstract »    Full Text »    PDF »
The Voltage-sensitive Release Mechanism of Excitation Contraction Coupling in Rabbit Cardiac Muscle Is Explained by Calcium-induced Calcium Release.
H. Griffiths and K.T. MacLeod (2003)
J. Gen. Physiol. 121, 353-373
   Abstract »    Full Text »    PDF »
Cardiac sodium-calcium exchanger: a double-edged sword.
S. J Conway and S. V Koushik (2001)
Cardiovasc Res 51, 194-197
   Full Text »    PDF »
Na+/Ca2+-exchange activity regulates contraction and SR Ca2+ content in rainbow trout atrial myocytes.
L. Hove-Madsen, A. Llach, and L. Tort (2000)
Am J Physiol Regulatory Integrative Comp Physiol 279, R1856-R1864
   Abstract »    Full Text »    PDF »
Sarcoplasmic Reticulum Ca2+ Release Causes Myocyte Depolarization : Underlying Mechanism and Threshold for Triggered Action Potentials.
K. Schlotthauer and D. M. Bers (2000)
Circ. Res. 87, 774-780
   Abstract »    Full Text »    PDF »
Progress in the understanding of cardiac early afterdepolarizations and torsades de pointes: time to revise current concepts.
P. G.A. Volders, M. A. Vos, B. Szabo, K. R. Sipido, S.H.M. de Groot, A. P.M. Gorgels, H. J.J. Wellens, and R. Lazzara (2000)
Cardiovasc Res 46, 376-392
   Full Text »    PDF »
Changes in Ca2+ Cycling Proteins Underlie Cardiac Action Potential Prolongation in a Pressure-Overloaded Guinea Pig Model With Cardiac Hypertrophy and Failure.
G. U. Ahmmed, P. H. Dong, G. Song, N. A. Ball, Y. Xu, R. A. Walsh, and N. Chiamvimonvat (2000)
Circ. Res. 86, 558-570
   Abstract »    Full Text »    PDF »
Intracellular Ca2+ oscillations drive spontaneous contractions in cardiomyocytes during early development.
S. Viatchenko-Karpinski, B. K. Fleischmann, Q. Liu, H. Sauer, O. Gryshchenko, G. J. Ji, and J. Hescheler (1999)
PNAS 96, 8259-8264
   Abstract »    Full Text »    PDF »
Sodium/Calcium Exchange: Its Physiological Implications.
M. P. Blaustein and W. J. Lederer (1999)
Physiol Rev 79, 763-854
   Abstract »    Full Text »    PDF »
Ca channels in cardiac myocytes: structure and function in Ca influx and intracellular Ca release.
D. M Bers and E. Perez-Reyes (1999)
Cardiovasc Res 42, 339-360
   Abstract »    Full Text »    PDF »
Overexpression of the Cardiac Na+/Ca2+ Exchanger Increases Susceptibility to Ischemia/Reperfusion Injury in Male, but Not Female, Transgenic Mice.
H. R. Cross, L. Lu, C. Steenbergen, K. D. Philipson, and E. Murphy (1998)
Circ. Res. 83, 1215-1223
   Abstract »    Full Text »    PDF »
Shortening and [Ca2+] dynamics of left ventricular myocytes isolated from exercise-trained rats.
B. M. Palmer, A. M. Thayer, S. M. Snyder, and R. L. Moore (1998)
J Appl Physiol 85, 2159-2168
   Abstract »    Full Text »    PDF »
Na+/Ca2+ exchange in neonatal rat heart cells: antisense inhibition and protein half-life.
M. K. Slodzinski and M. P. Blaustein (1998)
Am J Physiol Cell Physiol 275, C459-C467
   Abstract »    Full Text »    PDF »
Control of Maximum Sarcoplasmic Reticulum Ca Load in Intact Ferret Ventricular Myocytes: Effects of Thapsigargin and Isoproterenol.
K. S. Ginsburg, C. R. Weber, and D. M. Bers (1998)
J. Gen. Physiol. 111, 491-504
   Abstract »    Full Text »    PDF »
Contribution of reverse-mode sodium-calcium exchange to contractions in failing human left ventricular myocytes.
J. A Mattiello, K. B Margulies, V. Jeevanandam, and S. R Houser (1998)
Cardiovasc Res 37, 424-431
   Abstract »    Full Text »    PDF »
The role of L-type Ca2+ current and Na+ current-stimulated Na/Ca exchange in triggering SR calcium release in guinea-pig cardiac ventricular myocytes.
A.M Evans and M.B Cannell (1997)
Cardiovasc Res 35, 294-302
   Abstract »    Full Text »    PDF »
Regulation of Spontaneous Transient Outward Potassium Currents in Human Coronary Arteries.
R. Bychkov, M. Gollasch, C. Ried, F. C. Luft, and H. Haller (1997)
Circulation 95, 503-510
   Abstract »    Full Text »
Evidence for Functional Relevance of an Enhanced Expression of the Na+-Ca2+ Exchanger in Failing Human Myocardium.
M. Flesch, R. H.G. Schwinger, F. Schiffer, K. Frank, M. Sudkamp, F. Kuhn-Regnier, G. Arnold, and M. Bohm (1996)
Circulation 94, 992-1002
   Abstract »    Full Text »
Ca2+ Influx During the Cardiac Action Potential in Guinea Pig Ventricular Myocytes.
C.J. Grantham and M.B. Cannell (1996)
Circ. Res. 79, 194-200
   Abstract »    Full Text »
Sodium current-induced release of calcium from cardiac sarcoplasmic reticulum.
N Leblanc and Hume JR (1990)
Science 248, 372-376
   Abstract »    PDF »
The relationship between charge movements associated with ICa and INa-Ca in cardiac myocytes.
J. Bridge, Smolley JR, and K. Spitzer (1990)
Science 248, 376-378
   Abstract »    PDF »
Relaxation of isolated ventricular cardiomyocytes by a voltage-dependent process.
J. Bridge, K. Spitzer, and P. Ershler (1988)
Science 241, 823-825
   Abstract »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)