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Science 4 December 1987:
Vol. 238. no. 4832, pp. 1408 - 1410
DOI: 10.1126/science.3500513
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Articles

Science, Vol 238, Issue 4832, 1408-1410
Copyright © 1987 by American Association for the Advancement of Science

articles

Epidermal-growth-factor-dependent transformation by a human EGF receptor proto-oncogene

TJ Velu, L Beguinot, WC Vass, MC Willingham, GT Merlino, I Pastan, and DR Lowy

Laboratory of Cellular Oncology, National Cancer Institute, Bethesda, MD 20892.

The epidermal growth factor (EGF) receptor gene EGFR has been placed in a retrovirus vector to examine the growth properties of cells that experimentally overproduce a full-length EGF receptor. NIH 3T3 cells transfected with the viral DNA or infected with the corresponding rescued retrovirus developed a fully transformed phenotype in vitro that required both functional EGFR expression and the presence of EGF in the growth medium. Cells expressing 4 x 10(5) EGF receptors formed tumors in nude mice, while control cells did not. Therefore, the EGFR retrovirus, which had a titer on NIH 3T3 cells that was greater than 10(7) focus-forming units per milliliter, can efficiently transfer and express this gene, and increased numbers of EGF receptors can contribute to the transformed phenotype.


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