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ArticlesCopyright © 1987 by American Association for the Advancement of Science
Generation of a hybrid sequence-specific single-stranded deoxyribonuclease
Department of Chemistry, University of California, Berkeley 94720.
The relatively nonspecific single-stranded deoxyribonuclease, staphylococcal nuclease, was selectively fused to an oligonucleotide binding site of defined sequence to generate a hybrid enzyme. A cysteine was substituted for Lys116 in the enzyme by oligonucleotide-directed mutagenesis and coupled to an oligonucleotide that contained a 3'-thiol. The resulting hybrid enzyme cleaved single-stranded DNA at sites adjacent to the oligonucleotide binding site.
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Science. ISSN 0036-8075 (print), 1095-9203 (online)
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