Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Site Tools

  • AAAS
  • Subscribe
  • Feedback

Site Search

Search Advanced

Science 27 November 1987:
Vol. 238. no. 4831, pp. 1280 - 1283
DOI: 10.1126/science.2446389
Prev | Table of Contents | Next

Articles

Science, Vol 238, Issue 4831, 1280-1283
Copyright © 1987 by American Association for the Advancement of Science

articles

Signal for attachment of a phospholipid membrane anchor in decay accelerating factor

IW Caras, GN Weddell, MA Davitz, V Nussenzweig, and DW Martin Jr

Genentech, Inc., South San Francisco, CA 94080.

Decay accelerating factor (DAF) belongs to a novel group of membrane proteins anchored to the cell surface by a glycophospholipid membrane anchor that is covalently attached to the carboxyl terminus of the protein. The last 37 amino acids of membrane DAF, when fused to the carboxyl terminus of a secreted protein, are sufficient to target the fusion protein to the plasma membrane by means of a glycophospholipid anchor. This approach provides a novel means of targeting proteins to the cell-surface membrane.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Increased Susceptibility to Complement Attack due to Down-Regulation of Decay-Accelerating Factor/CD55 in Dysferlin-Deficient Muscular Dystrophy.
K. Wenzel, J. Zabojszcza, M. Carl, S. Taubert, A. Lass, C. L. Harris, M. Ho, H. Schulz, O. Hummel, N. Hubner, et al. (2005)
J. Immunol. 175, 6219-6225
   Abstract »    Full Text »    PDF »
Endoplasmic Reticulum-Localized Human Papillomavirus Type 16 E5 Protein Alters Endosomal pH but Not trans-Golgi pH.
G. L. Disbrow, J. A. Hanover, and R. Schlegel (2005)
J. Virol. 79, 5839-5846
   Abstract »    Full Text »    PDF »
Identification of GPI anchor attachment signals by a Kohonen self-organizing map.
N. Fankhauser and P. Maser (2005)
Bioinformatics 21, 1846-1852
   Abstract »    Full Text »    PDF »
Pathogenesis of Afa/Dr Diffusely Adhering Escherichia coli.
A. L. Servin (2005)
Clin. Microbiol. Rev. 18, 264-292
   Abstract »    Full Text »    PDF »
Removal of chylomicron remnants in transgenic mice overexpressing normal and membrane-anchored hepatic lipase.
S.-J. Lee, S. Kadambi, K. C-W. Yu, C. David, S. Azhar, A. D. Cooper, and S. Y. Choi (2005)
J. Lipid Res. 46, 27-35
   Abstract »    Full Text »    PDF »
The Membrane-Proximal Region of Vesicular Stomatitis Virus Glycoprotein G Ectodomain Is Critical for Fusion and Virus Infectivity.
E. Jeetendra, K. Ghosh, D. Odell, J. Li, H. P. Ghosh, and M. A. Whitt (2003)
J. Virol. 77, 12807-12818
   Abstract »    Full Text »    PDF »
Identification and study of a Candida albicans protein homologous to Saccharomyces cerevisiae Ssr1p, an internal cell-wall protein.
A. Garcera, A. I. Martinez, L. Castillo, M. V. Elorza, R. Sentandreu, and E. Valentin (2003)
Microbiology 149, 2137-2145
   Abstract »    Full Text »    PDF »
FGF3 Attached to a Phosholipid Membrane Anchor Gains a High Transforming Capacity. IMPLICATIONS OF MICRODOMAINS FOR FGF3 CELL TRANSFORMATION.
R. Kohl, M. Antoine, K. Reimers, and P. Kiefer (2002)
J. Biol. Chem. 277, 32760-32767
   Abstract »    Full Text »    PDF »
Intracellular Retention of Glycosylphosphatidyl Inositol-Linked Proteins in Caveolin-Deficient Cells.
F. Sotgia, B. Razani, G. Bonuccelli, W. Schubert, M. Battista, H. Lee, F. Capozza, A. L. Schubert, C. Minetti, J. T. Buckley, et al. (2002)
Mol. Cell. Biol. 22, 3905-3926
   Abstract »    Full Text »    PDF »
Functional Glycosylphosphatidylinositol Anchor Signal Sequences in the Pneumocystis carinii PRT1 Protease Family.
R. J. Palmer and A. E. Wakefield (2001)
Am. J. Respir. Cell Mol. Biol. 25, 466-473
   Abstract »    Full Text »    PDF »
The GPI Transamidase Complex of Saccharomyces cerevisiae Contains Gaa1p, Gpi8p, and Gpi16p.
P. Fraering, I. Imhof, U. Meyer, J.-M. Strub, A. van Dorsselaer, C. Vionnet, and A. Conzelmann (2001)
Mol. Biol. Cell 12, 3295-3306
   Abstract »    Full Text »    PDF »
Glycosyl-Phosphatidylinositol Reanchoring Unmasks Distinct Antigen-Presenting Pathways for CD1b and CD1c.
D. H. Geho, J. D. Fayen, R. M. Jackman, D. B. Moody, S. A. Porcelli, and M. L. Tykocinski (2000)
J. Immunol. 165, 1272-1277
   Abstract »    Full Text »    PDF »
Expression of Endogenously Activated Secreted or Cell Surface Carboxypeptidase A Sensitizes Tumor Cells to Methotrexate-{{alpha}}-Peptide Prodrugs.
D. A. Hamstra, M. Pagé, J. Maybaum, and A. Rehemtulla (2000)
Cancer Res. 60, 657-665
   Abstract »    Full Text »
Determination of the Affinity and Kinetic Constants for the Interaction between the Human Virus Echovirus 11 and Its Cellular Receptor, CD55.
S. M. Lea, R. M. Powell, T. McKee, D. J. Evans, D. Brown, D. I. Stuart, and P. A. van der Merwe (1998)
J. Biol. Chem. 273, 30443-30447
   Abstract »    Full Text »    PDF »
PRIM: Proximity imaging of green fluorescent protein-tagged polypeptides.
D. A. De Angelis, G. Miesenbock, B. V. Zemelman, and J. E. Rothman (1998)
PNAS 95, 12312-12316
   Abstract »    Full Text »    PDF »
A Retention Signal Necessary and Sufficient for Endoplasmic Reticulum Localization Maps to the Transmembrane Domain of Hepatitis C Virus Glycoprotein E2.
L. Cocquerel, J.-C. Meunier, A. Pillez, C. Wychowski, and J. Dubuisson (1998)
J. Virol. 72, 2183-2191
   Abstract »    Full Text »    PDF »
A family of secreted proteins contains homology to the cysteine-rich ligand-binding domain of frizzled receptors.
A. Rattner, J.-C. Hsieh, P. M. Smallwood, D. J. Gilbert, N. G. Copeland, N. A. Jenkins, and J. Nathans (1997)
PNAS 94, 2859-2863
   Abstract »    Full Text »    PDF »
Cell-surface Expression of an Amino-terminal Fragment of Apolipoprotein B Increases Lipoprotein Lipase Binding to Cells.
L. Pang, P. Sivaram, and I. J. Goldberg (1996)
J. Biol. Chem. 271, 19518-19523
   Abstract »    Full Text »    PDF »
Chinese Hamster Ovary Cells Expressing a Cell Surface-anchored Form of Hepatic Lipase. CHARACTERIZATION OF LOW DENSITY LIPOPROTEIN AND CHYLOMICRON REMNANT UPTAKE AND SELECTIVE UPTAKE OF HIGH DENSITY LIPOPROTEIN-CHOLESTERYL ESTER.
M. Komaromy, S. Azhar, and A. D. Cooper (1996)
J. Biol. Chem. 271, 16906-16914
   Abstract »    Full Text »    PDF »
Carboxyl terminus structural requirements for glycosyl-phosphatidylinositol anchor addition to cell surface proteins.
C Beghdadi-Rais, M Schreyer, M Rousseaux, P Borel, R. Eisenberg, G. Cohen, C Bron, and N Fasel (1993)
J. Cell Sci. 105, 831-840
   Abstract »    PDF »
Correction of a defect in mammalian GPI anchor biosynthesis by a transfected yeast gene.
R DeGasperi, L. Thomas, E Sugiyama, H. Chang, P. Beck, P Orlean, C Albright, G Waneck, J. Sambrook, C. Warren, et al. (1990)
Science 250, 988-991
   Abstract »    PDF »
Expression of T cell antigen receptor heterodimers in a lipid-linked form.
A. Lin, B Devaux, A Green, C Sagerstrom, J. Elliott, and M. Davis (1990)
Science 249, 677-679
   Abstract »    PDF »
Signal peptide for protein secretion directing glycophospholipid membrane anchor attachment.
I. Caras and G. Weddell (1989)
Science 243, 1196-1198
   Abstract »    PDF »
Structural and functional roles of glycosyl-phosphatidylinositol in membranes.
M. Low and A. Saltiel (1988)
Science 239, 268-275
   Abstract »    PDF »


ADVERTISEMENT
Click Me!

ADVERTISEMENT
Click Me!

To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)