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Science 13 November 1987:
Vol. 238. no. 4829, pp. 960 - 963
DOI: 10.1126/science.2823388
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Articles

Science, Vol 238, Issue 4829, 960-963
Copyright © 1987 by American Association for the Advancement of Science

articles

Transposon tagging and molecular analysis of the maize regulatory locus opaque-2

RJ Schmidt, FA Burr, and B Burr

Biology Department, Brookhaven National Laboratory, Upton, NY 11973.

Genetic analyses suggested that the opaque-2 (o2) locus in maize acts as a positive, transacting, transcriptional activator of the zein seed storage-protein genes. Because isolation of the gene is requisite to understanding the molecular details of this regulation, transposon mutagenesis with the transposable element suppressor-mutator (Spm) was carried out, and three mutable o2 alleles were obtained. One of these alleles contained an 8.3-kilobase autonomous Spm, another a 6.8-kilobase nonautonomous Spm, and the third an unidentified transposon that is unrelated to Spm. A DNA sequence flanking the autonomous Spm insertion was verified to be o2-specific and provided a probe to clone a wild-type allele. Northern blots indicated that the gene is expressed in wild-type endosperm but not in leaf tissues or in endosperms homozygous for a mutant allele of the O2 gene. A transcript was detected in endosperms homozygous for mutations at opaque-7 and floury-2, an indication that O2 expression is independent of these two other putative regulators of zein synthesis.


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