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Science 30 October 1987:
Vol. 238. no. 4827, pp. 645 - 650
DOI: 10.1126/science.3118463
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Articles

Science, Vol 238, Issue 4827, 645-650
Copyright © 1987 by American Association for the Advancement of Science

articles

Sequence-specific cleavage of double helical DNA by triple helix formation

HE Moser and PB Dervan

Division of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena 91125.

Homopyrimidine oligodeoxyribonucleotides with EDTA-Fe attached at a single position bind the corresponding homopyrimidine-homopurine tracts within large double-stranded DNA by triple helix formation and cleave at that site. Oligonucleotides with EDTA.Fe at the 5' end cause a sequence specific double strand break. The location and asymmetry of the cleavage pattern reveal that the homopyrimidine-EDTA probes bind in the major groove parallel to the homopurine strand of Watson-Crick double helical DNA. The sequence-specific recognition of double helical DNA by homopyrimidine probes is sensitive to single base mismatches. Homopyrimidine probes equipped with DNA cleaving moieties could be useful tools for mapping chromosomes.


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Science. ISSN 0036-8075 (print), 1095-9203 (online)