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Science 23 October 1987:
Vol. 238. no. 4826, pp. 514 - 517
DOI: 10.1126/science.3659925
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Articles

Science, Vol 238, Issue 4826, 514-517
Copyright © 1987 by American Association for the Advancement of Science

articles

X-ray holograms at improved resolution: a study of zymogen granules

M Howells, C Jacobsen, J Kirz, R Feder, K McQuaid, and S Rothman

Center for X-ray Optics, Lawrence Berkeley Laboratory, University of California, Berkeley 94720.

X-ray holography offers the possibility of three-dimensional microscopy with resolution higher than that of the light microscope and with contrast based on x-ray edges. In principle, the method is especially advantageous for biological samples if x-rays in the wavelength region between the carbon and oxygen K edges are used. However, until now the achieved resolution has not exceeded that of the light microscope because of the poor coherence properties of the x-ray sources and the low resolution of the detectors that were available. With a recently developed x-ray source based on an undulator on an electron storage ring, and high resolution x-ray resist, a hologram has been recorded at about 400-angstrom resolution. The experiment utilized x-rays with wavelengths of 24.7 angstroms and required a 1-hour exposure of the pancreatic zymogen granules under study.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Generation of Coherent X-rays in the Water Window Using 5-Femtosecond Laser Pulses.
C. Spielmann, N. H. Burnett, S. Sartania, R. Koppitsch, M. Schnürer, C. Kan, M. Lenzner, P. Wobrauschek, and F. Krausz (1997)
Science 278, 661-664
   Abstract »    Full Text »
High-Resolution Imaging by Fourier Transform X-ray Holography.
I. McNulty, J. Kirz, C. Jacobsen, E. H. Anderson, M. R. Howells, and D. P. Kern (1992)
Science 256, 1009-1012
   Abstract »    PDF »
Demonstration of X-ray Holography with an X-ray Laser.
J. E. TREBES, S. B. BROWN, E. M. CAMPBELL, D. L. MATTHEWS, D. G. NILSON, G. F. STONE, and D. A. WHELAN (1987)
Science 238, 517-519
   Abstract »    PDF »


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Science. ISSN 0036-8075 (print), 1095-9203 (online)