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Science 18 September 1987:
Vol. 237. no. 4821, pp. 1476 - 1479
DOI: 10.1126/science.3629250
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Articles

Science, Vol 237, Issue 4821, 1476-1479
Copyright © 1987 by American Association for the Advancement of Science

articles

Expression of an exogenous growth hormone gene by transplantable human epidermal cells

Morgan JR, Y Barrandon, H Green, and RC Mulligan

Retrovirus-mediated gene transfer was used to introduce a recombinant human growth hormone gene into cultured human keratinocytes. The transduced keratinocytes secreted biologically active growth hormone into the culture medium. When grafted as an epithelial sheet onto athymic mice, these cultured keratinocytes reconstituted an epidermis that was similar in appearance to that resulting from normal cells, but from which human growth hormone could be extracted. Transduced epidermal cells may prove to be a general vehicle for the delivery of gene products by means of grafting.


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