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Science 14 August 1987:
Vol. 237. no. 4816, pp. 749 - 753
DOI: 10.1126/science.2441470
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Articles

Science, Vol 237, Issue 4816, 749-753
Copyright © 1987 by American Association for the Advancement of Science

articles

Cloning of genomic and complementary DNA from Shaker, a putative potassium channel gene from Drosophila

DM Papazian, TL Schwarz, BL Tempel, YN Jan, and LY Jan

On the basis of electrophysiological analysis of Shaker mutants, the Shaker locus of Drosophila melanogaster has been proposed to encode a structural component of a voltage-dependent potassium channel, the A channel. Unlike sodium channels, acetylcholine receptors, and calcium channels, K+ channels have not been purified biochemically. To facilitate biochemical studies of a K+ channel, genomic DNA from the Shaker locus has been cloned. Rearrangements in five Shaker mutants have been mapped to a 60-kilobase segment of the genome. Four complementary DNA clones have been analyzed. These clones indicate that the Shaker gene contains multiple exons distributed over at least 65 kilobases of genomic DNA in the region where the mutations mapped. Furthermore, the gene may produce several classes of alternatively spliced transcripts. Two of the complementary DNA clones have been sequenced and their sequences support the hypothesis that Shaker encodes a component of a K+ channel.


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Molecular basis of gating charge immobilization in Shaker potassium channels.
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A component of calcium-activated potassium channels encoded by the Drosophila slo locus.
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Cold Spring Harb Symp Quant Biol 55, 9-17
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Mutant potassium channels with altered binding of charybdotoxin, a pore-blocking peptide inhibitor.
R MacKinnon and C Miller (1989)
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Expression of a cloned rat brain potassium channel in Xenopus oocytes.
M. Christie, J. Adelman, J Douglass, and R. North (1989)
Science 244, 221-224
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Cloning and expression of a Xenopus embryonic gap junction protein.
L Ebihara, E. Beyer, K. Swenson, D. Paul, and D. Goodenough (1989)
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