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Science 31 July 1987:
Vol. 237. no. 4814, pp. 525 - 527
DOI: 10.1126/science.3603039
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Articles

Science, Vol 237, Issue 4814, 525-527
Copyright © 1987 by American Association for the Advancement of Science

articles

A defined medium for a fastidious Spiroplasma

KJ Hackett, AS Ginsberg, S Rottem, RB Henegar, and RF Whitcomb

A defined medium (H-1) was developed for cultivation of the suckling mouse cataract agent, Spiroplasma mirum, a fastidious member of the class Mollicutes that causes cataracts and chronic brain infection in inoculated neonate mice. The H-1 medium was used to show the importance of sphingomyelin as a growth factor for the culture of the spiroplasma in vitro. The growth of Spiroplasma mirum and the pathology it induces in sphingomyelin-rich tissues in vivo may be related to this dependency.




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Science. ISSN 0036-8075 (print), 1095-9203 (online)