Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Site Tools

  • AAAS
  • Subscribe
  • Feedback

Site Search

Search Advanced

Science 22 May 1987:
Vol. 236. no. 4804, pp. 952 - 954
DOI: 10.1126/science.3576211
Prev | Table of Contents | Next

Articles

Science, Vol 236, Issue 4804, 952-954
Copyright © 1987 by American Association for the Advancement of Science

articles

Genetic analysis of halothane sensitivity in Caenorhabditis elegans

MM Sedensky and PM Meneely

The nematode Caenorhabditis elegans appears to be a useful model for studying the action of volatile anesthetics. A mutant strain that is hypersensitive to the widely used anesthetic halothane was described earlier. The mutation is now shown to be an allele of unc-79. Other alleles of unc-79 are also associated with hypersensitivity to halothane. A strain with a mutation in a second gene, unc-80, is also hypersensitive to halothane. Nematodes bearing mutations in both unc-79 and unc-80 are slightly more sensitive to halothane than those bearing only one of these mutations. Mutations in a third gene, unc-9, suppress both unc-79 and unc-80. Nematodes bearing the suppressor mutations alone have normal sensitivity to halothane. These results show that sensitivity to halothane can be altered by mutations in several different genes.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Low Conductance Gap Junctions Mediate Specific Electrical Coupling in Body-wall Muscle Cells of Caenorhabditis elegans.
Q. Liu, B. Chen, E. Gaier, J. Joshi, and Z.-W. Wang (2006)
J. Biol. Chem. 281, 7881-7889
   Abstract »    Full Text »    PDF »
Structural basis for high-affinity volatile anesthetic binding in a natural 4-helix bundle protein.
R. Liu, P. J. Loll, and R. G. Eckenhoff (2005)
FASEB J 19, 567-576
   Abstract »    Full Text »    PDF »
Resistance to Volatile Anesthetics by Mutations Enhancing Excitatory Neurotransmitter Release in Caenorhabditis elegans.
A. H. Hawasli, O. Saifee, C. Liu, M. L. Nonet, and C. M. Crowder (2004)
Genetics 168, 831-843
   Abstract »    Full Text »    PDF »
Nitrous oxide (N2O) requires the N-methyl-D-aspartate receptor for its action in Caenorhabditis elegans.
P. Nagele, L. B. Metz, and C. M. Crowder (2004)
PNAS 101, 8791-8796
   Abstract »    Full Text »    PDF »
Understanding anesthesia: making genetic sense of the absence of senses.
J. A. Humphrey, M. M. Sedensky, and P. G. Morgan (2002)
Hum. Mol. Genet. 11, 1241-1249
   Abstract »    Full Text »    PDF »
Effects of Nonimmobilizers and Halothane on Caenorhabditis elegans.
P. G. Morgan, G. W. Radke, and M. M. Sedensky (2000)
Anesth. Analg. 91, 1007-1012
   Abstract »    Full Text »    PDF »
SEL-5, A Serine/Threonine Kinase That Facilitates lin-12 Activity in Caenorhabditis elegans.
H. Fares and I. Greenwald (1999)
Genetics 153, 1641-1654
   Abstract »    Full Text »
A Stomatin and a Degenerin Interact to Control Anesthetic Sensitivity in Caenorhabditis elegans.
S. Rajaram, T. L. Spangler, M. M. Sedensky, and P. G. Morgan (1999)
Genetics 153, 1673-1682
   Abstract »    Full Text »


ADVERTISEMENT
Click Me!

ADVERTISEMENT
Click Me!

To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)