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Science 22 May 1987:
Vol. 236. no. 4804, pp. 952 - 954
DOI: 10.1126/science.3576211
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Articles

Science, Vol 236, Issue 4804, 952-954
Copyright © 1987 by American Association for the Advancement of Science

articles

Genetic analysis of halothane sensitivity in Caenorhabditis elegans

MM Sedensky and PM Meneely

The nematode Caenorhabditis elegans appears to be a useful model for studying the action of volatile anesthetics. A mutant strain that is hypersensitive to the widely used anesthetic halothane was described earlier. The mutation is now shown to be an allele of unc-79. Other alleles of unc-79 are also associated with hypersensitivity to halothane. A strain with a mutation in a second gene, unc-80, is also hypersensitive to halothane. Nematodes bearing mutations in both unc-79 and unc-80 are slightly more sensitive to halothane than those bearing only one of these mutations. Mutations in a third gene, unc-9, suppress both unc-79 and unc-80. Nematodes bearing the suppressor mutations alone have normal sensitivity to halothane. These results show that sensitivity to halothane can be altered by mutations in several different genes.


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Structural basis for high-affinity volatile anesthetic binding in a natural 4-helix bundle protein.
R. Liu, P. J. Loll, and R. G. Eckenhoff (2005)
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Resistance to Volatile Anesthetics by Mutations Enhancing Excitatory Neurotransmitter Release in Caenorhabditis elegans.
A. H. Hawasli, O. Saifee, C. Liu, M. L. Nonet, and C. M. Crowder (2004)
Genetics 168, 831-843
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Nitrous oxide (N2O) requires the N-methyl-D-aspartate receptor for its action in Caenorhabditis elegans.
P. Nagele, L. B. Metz, and C. M. Crowder (2004)
PNAS 101, 8791-8796
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Understanding anesthesia: making genetic sense of the absence of senses.
J. A. Humphrey, M. M. Sedensky, and P. G. Morgan (2002)
Hum. Mol. Genet. 11, 1241-1249
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Effects of Nonimmobilizers and Halothane on Caenorhabditis elegans.
P. G. Morgan, G. W. Radke, and M. M. Sedensky (2000)
Anesth. Analg. 91, 1007-1012
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SEL-5, A Serine/Threonine Kinase That Facilitates lin-12 Activity in Caenorhabditis elegans.
H. Fares and I. Greenwald (1999)
Genetics 153, 1641-1654
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A Stomatin and a Degenerin Interact to Control Anesthetic Sensitivity in Caenorhabditis elegans.
S. Rajaram, T. L. Spangler, M. M. Sedensky, and P. G. Morgan (1999)
Genetics 153, 1673-1682
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