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Science 13 March 1987:
Vol. 235. no. 4794, pp. 1387 - 1390
DOI: 10.1126/science.3029868
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Articles

Science, Vol 235, Issue 4794, 1387-1390
Copyright © 1987 by American Association for the Advancement of Science

articles

Megabase-scale mapping of the HLA gene complex by pulsed field gel electrophoresis

SK Lawrance, CL Smith, R Srivastava, CR Cantor, and SM Weissman

In the study of the genetic structure of mammalian chromosomes, there exists a "resolution gap" between molecular cloning experiments and meiotic linkage analyses. This gap has discouraged attempts to construct full-scale genetic maps of mammalian chromosomes. The organization of the human major histocompatibility complex was examined within this range by pulsed field gel electrophoresis. The data obtained indicate that the complex spans over 3000 kilobases and enable the construction of a megabase-scale molecular map. These results indicate that the techniques employed in DNA extraction, enzymatic digestion, electrophoresis, and hybridization are suitable for the efficient analysis of megabase regions of mammalian chromosomes and effectively bridge the resolution gap between molecular cloning and classical genetics.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
A new cluster of genes within the human major histocompatibility complex.
T Spies, G Blanck, M Bresnahan, J Sands, and J. Strominger (1989)
Science 243, 214-217
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