Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Science 19 December 1986:
Vol. 234. no. 4783, pp. 1570 - 1573
DOI: 10.1126/science.3787262
Prev | Table of Contents | Next

Articles

Science, Vol 234, Issue 4783, 1570-1573
Copyright © 1986 by American Association for the Advancement of Science

articles

Selective chemical catalysis by an antibody

SJ Pollack, JW Jacobs, and PG Schultz

The immunoglobulin MOPC167, which binds the transition state analog p-nitrophenylphosphorylcholine with high affinity, catalyzed the hydrolysis of the corresponding carbonate 1. MOPC167 catalysis displayed saturation kinetics with catalytic constant (kcat) = 0.4 min-1 and Michaelis constant (Km) = 208 microM, showed substrate specificity, and was inhibited by p-nitrophenylphosphorylcholine. The rate of the reaction was first order in hydroxide ion concentration between pH 6.0 and 8.0. The lower limit for the rate of acceleration of hydrolysis by the antibody above the uncatalyzed reaction was 770. This study begins to define the rules for the generation of catalytic antibodies.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Identification of anti-prothrombin antibodies in the anti-phospholipid syndrome that display the prothrombinase activity.
Y.-H. Yang, C.-J. Chang, Y.-H. Chuang, H.-Y. Hsu, P. P. Chen, and B.-L. Chiang (2010)
Rheumatology 49, 34-42
   Abstract »    Full Text »    PDF »
Mechanistic Analysis of the Phosphonate Transition-state Analogue-derived Catalytic and Non-catalytic Antibody.
Y. Nishi, N. Yamamoto, K. Shimazaki, N. Takahashi-Ando, H. Kakinuma, S. Jialin, S. N. Ruzheinikov, T. A. Muranova, D. W. Rice, and Y. Kajihara (2007)
J. Biochem. 142, 421-433
   Abstract »    Full Text »    PDF »
Conformational Transitions as Determinants of Specificity for the DNA Methyltransferase EcoRI.
B. Youngblood and N. O. Reich (2006)
J. Biol. Chem. 281, 26821-26831
   Abstract »    Full Text »    PDF »
Incorporation of a single His residue by rational design enables thiol-ester hydrolysis by human glutathione transferase A1-1.
S. Hederos, K. S. Broo, E. Jakobsson, G. J. Kleywegt, B. Mannervik, and L. Baltzer (2004)
PNAS 101, 13163-13167
   Abstract »    Full Text »    PDF »
Prodrugs of dynemicin analogs for selective chemotherapy mediated by an aldolase catalytic Ab.
S. C. Sinha, L.-S. Li, G. P. Miller, S. Dutta, C. Rader, and R. A. Lerner (2004)
PNAS 101, 3095-3099
   Abstract »    Full Text »    PDF »
Enzyme-like proteins from an unselected library of designed amino acid sequences.
Y. Wei and M. H. Hecht (2004)
Protein Eng. Des. Sel. 17, 67-75
   Abstract »    Full Text »    PDF »
Production of a Functional Catalytic Antibody ScFv-NusA Fusion Protein in Bacterial Cytoplasm.
L. Zheng, U. Baumann, and J.-L. Reymond (2003)
J. Biochem. 133, 577-581
   Abstract »    Full Text »    PDF »
Structural evidence for substrate strain in antibody catalysis.
J. Yin, S. E. Andryski, A. E. Beuscher IV, R. C. Stevens, and P. G. Schultz (2003)
PNAS 100, 856-861
   Abstract »    Full Text »    PDF »
Molecular Evolution of Catalytic Antibodies in Autoimmune Mice.
J. Sun, N. Takahashi, H. Kakinuma, and Y. Nishi (2001)
J. Immunol. 167, 5775-5785
   Abstract »    Full Text »    PDF »
Toward antibody-catalyzed hydrolysis of organophosphorus poisons.
P. Vayron, P.-Y. Renard, F. Taran, C. Creminon, Y. Frobert, J. Grassi, and C. Mioskowski (2000)
PNAS 97, 7058-7063
   Abstract »    Full Text »    PDF »
Adaptive Recognition by Nucleic Acid Aptamers.
T. Hermann and D. J. Patel (2000)
Science 287, 820-825
   Abstract »    Full Text »
Bringing biological solutions to chemical problems.
P. G. Schultz (1998)
PNAS 95, 14590-14591
   Full Text »    PDF »
The antibody catalysis route to the total synthesis of epothilones.
S. C. Sinha, C. F. Barbas III, and R. A. Lerner (1998)
PNAS 95, 14603-14608
   Abstract »    Full Text »    PDF »
Functionalized Protein-like Structures from Conformationally Defined Synthetic Combinatorial Libraries.
E. Pérez-Payá, R. A. Houghten, and S. E. Blondelle (1996)
J. Biol. Chem. 271, 4120-4126
   Abstract »    Full Text »    PDF »
From molecular diversity to catalysis: lessons from the immune system.
P. Schultz and R. Lerner (1995)
Science 269, 1835-1842
   Abstract »    PDF »
A Combinatorial Approach to Materials Discovery.
X. -D. Xiang, X. Sun, G. Briceno, Y. Lou, K.-A. Wang, H. Chang, W. G. Wallace-Freedman, S.-W. Chen, and P. G. Schultz (1995)
Science 268, 1738-1740
   Abstract »    PDF »
Crystal structure of a catalytic antibody with a serine protease active site.
G. Zhou, J Guo, W Huang, R. Fletterick, and T. Scanlan (1994)
Science 265, 1059-1064
   Abstract »    PDF »
Expanding the scope of RNA catalysis.
Prudent JR, T Uno, and P. Schultz (1994)
Science 264, 1924-1927
   Abstract »    PDF »
Antibody-catalyzed degradation of cocaine.
D. Landry, K Zhao, G. Yang, M Glickman, and T. Georgiadis (1993)
Science 259, 1899-1901
   Abstract »    PDF »
At the crossroads of chemistry and immunology: catalytic antibodies.
R. Lerner, S. Benkovic, and P. Schultz (1991)
Science 252, 659-667
   Abstract »    PDF »
Antibody-catalyzed porphyrin metallation.
A. Cochran and P. Schultz (1990)
Science 249, 781-783
   Abstract »    PDF »
Generation of a large combinatorial library of the immunoglobulin repertoire in phage lambda.
W. Huse, L Sastry, S. Iverson, A. Kang, M Alting-Mees, D. Burton, S. Benkovic, and R. Lerner (1989)
Science 246, 1275-1281
   Abstract »    PDF »
Enzymatic catalysts in organic synthesis.
C. Wong (1989)
Science 244, 1145-1152
   Abstract »    PDF »
Catalytic hydrolysis of vasoactive intestinal peptide by human autoantibody.
S Paul, D. Volle, C. Beach, D. Johnson, M. Powell, and R. Massey (1989)
Science 244, 1158-1162
   Abstract »    PDF »
Catalytic antibodies with lipase activity and R or S substrate selectivity.
K. Janda, S. Benkovic, and R. Lerner (1989)
Science 244, 437-440
   Abstract »    PDF »
Sequence-specific peptide cleavage catalyzed by an antibody.
B. Iverson and R. Lerner (1989)
Science 243, 1184-1188
   Abstract »    PDF »
A Combinatorial System for Cloning and Expressing the Catalytic Antibody Repertoire in Escherichia coli.
S.A. Iverson, L. Sastry, W.D. Huse, J.A. Sorge, S.J. Benkovic, and R.A. Lerner (1989)
Cold Spring Harb Symp Quant Biol 54, 273-281
   Abstract »    PDF »
Introduction of nucleophiles and spectroscopic probes into antibody combining sites.
S. Pollack, G. Nakayama, and P. Schultz (1988)
Science 242, 1038-1040
   Abstract »    PDF »
How do enzymes work?.
J Kraut (1988)
Science 242, 533-540
   Abstract »    PDF »
Induction of an antibody that catalyzes the hydrolysis of an amide bond.
K. Janda, D Schloeder, S. Benkovic, and R. Lerner (1988)
Science 241, 1188-1191
   Abstract »    PDF »
Assembly of a functional immunoglobulin Fv fragment in Escherichia coli.
A Skerra and A Pluckthun (1988)
Science 240, 1038-1041
   Abstract »    PDF »
The interplay between chemistry and biology in the design of enzymatic catalysts.
P. Schultz (1988)
Science 240, 426-433
   Abstract »    PDF »
A stereospecific cyclization catalyzed by an antibody.
A. Napper, S. Benkovic, A Tramontano, and R. Lerner (1987)
Science 237, 1041-1043
   Abstract »    PDF »
Antibody Catalysis by Transition State Stabilization.
S.J. Pollack and P.G. Schultz (1987)
Cold Spring Harb Symp Quant Biol 52, 97-104
   Abstract »    PDF »
Engineering of Antibodies with a Known Three-dimensional Structure.
A. Pluckthun, R. Glockshuber, I. Pfitzinger, A. Skerra, and J. Stadlmuller (1987)
Cold Spring Harb Symp Quant Biol 52, 105-112
   Abstract »    PDF »
Inaugural Article: Cyclic peptide formation catalyzed by an antibody ligase.
D. B. Smithrud, P. A. Benkovic, S. J. Benkovic, V. Roberts, J. Liu, I. Neagu, S. Iwama, B. W. Phillips, A. B. Smith III, and R. Hirschmann (2000)
PNAS 97, 1953-1958
   Abstract »    Full Text »    PDF »
Enzyme mimicry by the antiidiotypic antibody approach.
A. V. Kolesnikov, A. V. Kozyr, E. S. Alexandrova, F. Koralewski, A. V. Demin, M. I. Titov, B. Avalle, A. Tramontano, S. Paul, D. Thomas, et al. (2000)
PNAS 97, 13526-13531
   Abstract »    Full Text »    PDF »
From the Cover: Enzyme-like proteins by computational design.
D. N. Bolon and S. L. Mayo (2001)
PNAS 98, 14274-14279
   Abstract »    Full Text »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)