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Science 19 December 1986:
Vol. 234. no. 4783, pp. 1566 - 1570
DOI: 10.1126/science.3787261
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Articles

Science, Vol 234, Issue 4783, 1566-1570
Copyright © 1986 by American Association for the Advancement of Science

articles

Catalytic antibodies

A Tramontano, KD Janda, and RA Lerner

Monoclonal antibodies elicited to haptens that are analogs of the transition state for hydrolysis of carboxylic esters behaved as enzymic catalysts with the appropriate substrates. These substrates are distinguished by the structural congruence of both hydrolysis products with haptenic fragments. The haptens were potent inhibitors of this esterolytic activity, in agreement with their classification as transition state analogs. Mechanisms are proposed to account for the different chemical behavior of these antibodies with two types of ester substrates. The generation of an artificial enzyme through transition state stabilization by antibodies was thus demonstrated. These studies indicate a potentially general approach to catalyst design.


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