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Science 12 December 1986:
Vol. 234. no. 4782, pp. 1409 - 1413
DOI: 10.1126/science.3024318
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Articles

Science, Vol 234, Issue 4782, 1409-1413
Copyright © 1986 by American Association for the Advancement of Science

articles

Tissue-specific and ectopic expression of genes introduced into transgenic mice by retroviruses

P Soriano, RD Cone, RC Mulligan, and R Jaenisch

Recombinant retroviruses containing the complete genomic human beta globin gene (under the control of its own promoter) and the bacterial neomycin phosphotransferase gene (under the control of the normal or enhancerless viral promoter) were used to derive transgenic mouse strains by infection of preimplantation embryos. Expression of the beta globin gene in hematopoietic tissues was observed in all transgenic strains. In addition, one strain showed ectopic expression of beta globin in the same tissues that also expressed high levels of RNA from the viral promoter. It is likely that expression from the long terminal repeat (LTR), in contrast to expression from the internal promoter, is dependent on the site of integration. Thus, retroviral vectors can be used for tissue-specific expression of foreign genes in transgenic mice, as well as for the identification of loci that allow developmental activation of a provirus.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Rhesus monkey placental transgene expression after lentiviral gene transfer into preimplantation embryos.
M. J. Wolfgang, S. G. Eisele, M. A. Browne, M. L. Schotzko, M. A. Garthwaite, M. Durning, A. Ramezani, R. G. Hawley, J. A. Thomson, and T. G. Golos (2001)
PNAS 98, 10728-10732
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Lentivirus Vector Gene Expression during ES Cell-Derived Hematopoietic Development In Vitro.
I. Hamaguchi, N.-B. Woods, I. Panagopoulos, E. Andersson, H. Mikkola, C. Fahlman, R. Zufferey, L. Carlsson, D. Trono, and S. Karlsson (2000)
J. Virol. 74, 10778-10784
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Host cis-Mediated Extinction of a Retrovirus Permissive for Expression in Embryonal Stem Cells during Differentiation.
C. Laker, J. Meyer, A. Schopen, J. Friel, C. Heberlein, W. Ostertag, and C. Stocking (1998)
J. Virol. 72, 339-348
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Germ-line gene modification and disease prevention: some medical and ethical perspectives.
N. Wivel and L Walters (1993)
Science 262, 533-538
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Promoter traps in embryonic stem cells: a genetic screen to identify and mutate developmental genes in mice..
G Friedrich and P Soriano (1991)
Genes & Dev. 5, 1513-1523
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Synthesis of functional human hemoglobin in transgenic mice.
R. Behringer, T. Ryan, M. Reilly, T Asakura, R. Palmiter, R. Brinster, and T. Townes (1989)
Science 245, 971-973
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Germline transmission of exogenous genes in the chicken.
R. Bosselman, R. Hsu, T Boggs, S Hu, J Bruszewski, S Ou, L Kozar, F Martin, C Green, F Jacobsen, et al. (1989)
Science 243, 533-535
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Transgenic animals.
R Jaenisch (1988)
Science 240, 1468-1474
   Abstract »    PDF »
Retroviruses and insertional mutagenesis in mice: proviral integration at the Mov 34 locus leads to early embryonic death..
P Soriano, T Gridley, and R Jaenisch (1987)
Genes & Dev. 1, 366-375
   Abstract »    PDF »


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