Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.
Science Career Fair

Site Tools

  • AAAS
  • Subscribe
  • Feedback

Site Search

Search Advanced

Science 12 December 1986:
Vol. 234. no. 4782, pp. 1388 - 1391
DOI: 10.1126/science.3787251
Prev | Table of Contents | Next

Articles

Science, Vol 234, Issue 4782, 1388-1391
Copyright © 1986 by American Association for the Advancement of Science

articles

Inhibition of secretion of hepatitis B surface antigen by a related presurface polypeptide

DH Persing, HE Varmus, and D Ganem

The presurface (preS) proteins of hepatitis B virus are structural components of the viral envelope that may play important roles in virion assembly and infectivity. They are specified by a large open reading frame that includes the coding region for the major surface (S) protein in its 3' half. Translation of the preS proteins initiates upstream from the S region, giving rise to proteins that are composed of the S domain and an additional 163 (preS1) or 55 (preS2) amino acids. Little is known about the biosynthesis and assembly of these proteins. The expression of the S and preS1 proteins was examined by transfecting cultured mammalian cells with viral DNA and injecting synthetic messenger RNA's into Xenopus oocytes. In contrast to the proteins encoded by the S region, the preS1 proteins are not detectably secreted into the culture medium. Furthermore, when the S and preS1 proteins are synthesized together, secretion of the S proteins is specifically and strongly inhibited. The results suggest a unique molecular interaction during secretion of the S and preS proteins that may be important for virus assembly.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Immunoadhesins Containing Pre-S Domains of Hepatitis B Virus Large Envelope Protein Are Secreted and Inhibit Virus Infection.
N. Chai, S. Gudima, J. Chang, and J. Taylor (2007)
J. Virol. 81, 4912-4918
   Abstract »    Full Text »    PDF »
Hepatitis B Virus Subviral Envelope Particle Morphogenesis and Intracellular Trafficking.
R. Patient, C. Hourioux, P.-Y. Sizaret, S. Trassard, C. Sureau, and P. Roingeard (2007)
J. Virol. 81, 3842-3851
   Abstract »    Full Text »    PDF »
Analysis of the Cytosolic Domains of the Hepatitis B Virus Envelope Proteins for Their Function in Viral Particle Assembly and Infectivity.
M. Blanchet and C. Sureau (2006)
J. Virol. 80, 11935-11945
   Abstract »    Full Text »    PDF »
Hepatitis B virus particle formation in the absence of pregenomic RNA and reverse transcriptase..
W. Schormann, A. Kraft, D. Ponsel, and V. Bruss (2006)
J. Virol. 80, 4187-4190
   Abstract »    Full Text »    PDF »
Reduced Secretion of Virions and Hepatitis B Virus (HBV) Surface Antigen of a Naturally Occurring HBV Variant Correlates with the Accumulation of the Small S Envelope Protein in the Endoplasmic Reticulum and Golgi Apparatus.
P. K. Chua, R. Y.-L. Wang, M.-H. Lin, T. Masuda, F.-M. Suk, and C. Shih (2005)
J. Virol. 79, 13483-13496
   Abstract »    Full Text »    PDF »
Novel Autoregulatory Function of Hepatitis B Virus M Protein on Surface Gene Expression.
T.-J. Huang, C.-C. Lu, J.-C. Tsai, W.-J. Yao, X. Lu, M.-D. Lai, H.-S. Liu, and A.-L. Shiau (2005)
J. Biol. Chem. 280, 27742-27754
   Abstract »    Full Text »    PDF »
Modulation of Hepatitis B Virus Secretion by Naturally Occurring Mutations in the S Gene.
N. Khan, M. Guarnieri, S. H. Ahn, J. Li, Y. Zhou, G. Bang, K.-H. Kim, J. R. Wands, and S. Tong (2004)
J. Virol. 78, 3262-3270
   Abstract »    Full Text »    PDF »
Different Types of Ground Glass Hepatocytes in Chronic Hepatitis B Virus Infection Contain Specific Pre-S Mutants that May Induce Endoplasmic Reticulum Stress.
H.-C. Wang, H.-C. Wu, C.-F. Chen, N. Fausto, H.-Y. Lei, and I.-J. Su (2003)
Am. J. Pathol. 163, 2441-2449
   Abstract »    Full Text »
Molecular Chaperone GRP78/BiP Interacts with the Large Surface Protein of Hepatitis B Virus In Vitro and In Vivo.
D.-Y. Cho, G.-H. Yang, C. J. Ryu, and H. J. Hong (2003)
J. Virol. 77, 2784-2788
   Abstract »    Full Text »    PDF »
Influence of a Putative Intermolecular Interaction between Core and the Pre-S1 Domain of the Large Envelope Protein on Hepatitis B Virus Secretion.
S. Le Pogam and C. Shih (2002)
J. Virol. 76, 6510-6517
   Abstract »    Full Text »    PDF »
A Short N-Proximal Region in the Large Envelope Protein Harbors a Determinant That Contributes to the Species Specificity of Human Hepatitis B Virus.
P. Chouteau, J. Le Seyec, I. Cannie, M. Nassal, C. Guguen-Guillouzo, and P. Gripon (2001)
J. Virol. 75, 11565-11572
   Abstract »    Full Text »
Evidence for structural differences in the S domain of L in comparison with S protein of hepatitis B virus.
R. F. Clayton, A. Owsianka, and A. H. Patel (2001)
J. Gen. Virol. 82, 1533-1541
   Abstract »    Full Text »    PDF »
Intracellular Retention of Hepatitis B Virus Surface Proteins Reduces Interleukin-2 Augmentation after Genetic Immunizations.
M. Geissler, V. Bruss, S. Michalak, B. Hockenjos, D. Ortmann, W. B. Offensperger, J. R. Wands, and H. E. Blum (1999)
J. Virol. 73, 4284-4292
   Abstract »    Full Text »
Infection Process of the Hepatitis B Virus Depends on the Presence of a Defined Sequence in the Pre-S1 Domain.
J. Le Seyec, P. Chouteau, I. Cannie, C. Guguen-Guillouzo, and P. Gripon (1999)
J. Virol. 73, 2052-2057
   Abstract »    Full Text »
Protective Efficacy of DNA Vaccines against Duck Hepatitis B Virus Infection.
M. Triyatni, A. R. Jilbert, M. Qiao, D. S. Miller, and C. J. Burrell (1998)
J. Virol. 72, 84-94
   Abstract »    Full Text »    PDF »


ADVERTISEMENT
Click Me!

ADVERTISEMENT
Click Me!

To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)