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Science 5 December 1986:
Vol. 234. no. 4781, pp. 1237 - 1240
DOI: 10.1126/science.3775382
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Articles

Science, Vol 234, Issue 4781, 1237-1240
Copyright © 1986 by American Association for the Advancement of Science

articles

Neuronal properties of clonal hybrid cell lines derived from central cholinergic neurons

DN Hammond, BH Wainer, JH Tonsgard, and A Heller

Clonal cell lines derived from specific types of central neurons can be used to identify and characterize properties specific to those neurons. With somatic cell fusion techniques, nine clonal hybrid cell lines have been developed from the septal region of the mouse basal forebrain. Two lines express characteristics typical of cholinergic neurons--choline acetyltransferase activity and immunoreactivity, neurite formation with neurofilament protein immunoreactivity, and aggregation in rotation-mediated cell culture. These cell lines may be useful for studying the trophic interactions that support the development and maintenance of central cholinergic connections.


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