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Science 28 November 1986:
Vol. 234. no. 4780, pp. 1117 - 1119
DOI: 10.1126/science.3465038
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Articles

Science, Vol 234, Issue 4780, 1117-1119
Copyright © 1986 by American Association for the Advancement of Science

articles

Pertussis toxin-sensitive pathway in the stimulation of c-myc expression and DNA synthesis by bombesin

JJ Letterio, Coughlin SR, and LT Williams

The bombesin-like peptides are potent mitogens for Swiss 3T3 fibroblasts, human bronchial epithelial cells, and cells isolated from small cell carcinoma of the lung. The mechanism of signal transduction in the proliferative response to bombesin was investigated by studying the effect of Bordetella pertussis toxin on bombesin-stimulated mitogenesis. At nanomolar concentrations, bombesin increased levels of c-myc messenger RNA and stimulated DNA synthesis in Swiss 3T3 cells. Treatment of the cells with pertussis toxin (5 nanograms per milliliter) completely blocked bombesin-enhanced c-myc expression and eliminated bombesin-stimulated DNA synthesis. This treatment had essentially no effect on the mitogenic responses to either platelet-derived growth factor or phorbol 12,13-dibutyrate. These results suggest that the mitogenic actions of bombesin-like growth factors are mediated through a pertussis toxin-sensitive guanine nucleotide-binding protein. Furthermore they indicate that bombesin-like growth factors act through pathways that are different from those activated by platelet-derived growth factor.


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