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Science 14 November 1986:
Vol. 234. no. 4778, pp. 863 - 866
DOI: 10.1126/science.3775367
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Articles

Science, Vol 234, Issue 4778, 863-866
Copyright © 1986 by American Association for the Advancement of Science

articles

Curvilinear, three-dimensional motion of chromatin domains and nucleoli in neuronal interphase nuclei

U De Boni and AH Mintz

The term "nuclear rotation" refers to a motion of nucleoli within interphase nuclei of several cell types. No mechanism or function has been ascribed to this phenomenon, and it was unknown whether nuclear structures in addition to nucleoli participate in this motion. Moreover, it was unclear whether nuclear rotation occurs independent of concurrent motion of juxtanuclear cytoplasm. The work reported here presents quantitative evidence, for three-dimensional intranuclear, tandem motion of fluorescently labeled chromatin domains associated with nucleoli and those remote from nucleoli. The results show that such motion is curvilinear, that it is not restricted to nucleoli, and, moreover, that it occurs independently of motion of juxtanuclear, cytoplasmic structures. These results suggest that this motion represents karyoplasmic streaming and its function is to transpose to nuclear pores those chromatin domains actively transcribed.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
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Chromosomes exhibit preferential positioning in nuclei of quiescent human cells.
R. Nagele, T Freeman, L McMorrow, Z Thomson, K Kitson-Wind, and H. Lee (1999)
J. Cell Sci. 112, 525-535
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Selective Entrapment of Extrachromosomally Amplified DNA by Nuclear Budding and Micronucleation during S Phase.
N. Shimizu, N. Itoh, H. Utiyama, and G. M. Wahl (1998)
J. Cell Biol. 140, 1307-1320
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DNA replication-dependent intranuclear relocation of double minute chromatin.
N Itoh and N Shimizu (1998)
J. Cell Sci. 111, 3275-3285
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Changes in Morphology and Spatial Position of Coiled Bodies during NGF-induced Neuronal Differentiation of PC12 Cells.
J. Janevski, P. C. Park, and U. De Boni (1997)
J. Histochem. Cytochem. 45, 1523-1532
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Chromatin Dynamics in Interphase Nuclei and Its Implications for Nuclear Structure.
J. R. Abney, B. Cutler, M. L. Fillbach, D. Axelrod, and B. A. Scalettar (1997)
J. Cell Biol. 137, 1459-1468
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In vivo observation of the puff-specific protein no-on transient A (NONA) in nuclei of Drosophila embryos.
P Buchenau, D. Arndt-Jovin, and H Saumweber (1993)
J. Cell Sci. 106, 189-199
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The relative positions of condensed chromosomes are maintained between divisions in the epidermis of Calpodes ethlius.
G. Jeun and M. Locke (1992)
J. Cell Sci. 103, 839-846
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A view of interphase chromosomes.
L Manuelidis (1990)
Science 250, 1533-1540
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Movement of the X chromosome in epilepsy.
J Borden and L Manuelidis (1988)
Science 242, 1687-1691
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