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Science 31 October 1986:
Vol. 234. no. 4776, pp. 593 - 596
DOI: 10.1126/science.3094144
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Articles

Science, Vol 234, Issue 4776, 593-596
Copyright © 1986 by American Association for the Advancement of Science

articles

Identification of paramyosin as schistosome antigen recognized by intradermally vaccinated mice

DE Lanar, EJ Pearce, SL James, and A Sher

Mice immunized intradermally with extracts of Schistosoma mansoni in combination with the adjuvant BCG are significantly protected against subsequent infection with living larval forms of the parasite. Remarkably, these vaccinated animals produce antibodies predominantly against a single parasite protein of molecular weight 97 kilodaltons (Sm-97). A complementary DNA that encodes about half of the Sm-97 molecule has now been cloned and sequenced. Analysis of the deduced amino acid sequence reveals a protein containing periodic repeats of hydrophobic amino acids characteristic of an alpha-helical coiled-coil structure. The deduced amino acid composition of the cloned gene and several properties of the native protein are similar to that of paramyosin, an alpha-helical protein that forms the core for myosin filaments in invertebrate muscle. Paramyosin was isolated from Schistosoma mansoni adult worms and antibodies to Sm-97 were shown to react with this molecule as well as with a known paramyosin from molluscan muscle.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Pilot-Scale Production and Characterization of Paramyosin, a Vaccine Candidate for Schistosomiasis Japonica.
M. Jiz, H.-W. Wu, R. Meng, S. Pond-Tor, M. Reynolds, J. F. Friedman, R. Olveda, L. Acosta, and J. D. Kurtis (2008)
Infect. Immun. 76, 3164-3169
   Abstract »    Full Text »    PDF »
Current Status of Vaccines for Schistosomiasis.
D. P. McManus and A. Loukas (2008)
Clin. Microbiol. Rev. 21, 225-242
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Invertebrate Muscles: Muscle Specific Genes and Proteins.
S. L. Hooper and J. B. Thuma (2005)
Physiol Rev 85, 1001-1060
   Abstract »    Full Text »    PDF »
Inhibition of the Complement Membrane Attack Complex by Schistosoma mansoni Paramyosin.
J. Deng, D. Gold, P. T. LoVerde, and Z. Fishelson (2003)
Infect. Immun. 71, 6402-6410
   Abstract »    Full Text »    PDF »
Induction of Protective Immunity against Schistosoma mansoni via DNA Priming and Boosting with the Large Subunit of Calpain (Sm-p80): Adjuvant Effects of Granulocyte-Macrophage Colony-Stimulating Factor and Interleukin-4.
A. A. Siddiqui, T. Phillips, H. Charest, R. B. Podesta, M. L. Quinlin, J. R. Pinkston, J. D. Lloyd, M. Paz, R. M. Villalovos, and J. Pompa (2003)
Infect. Immun. 71, 3844-3851
   Abstract »    Full Text »    PDF »
Identification of Paramyosin as a Binding Protein for Calgranulin C in Experimental Helminthic Keratitis.
E. K. Akpek, S. H. Liu, R. Thompson, and J. D. Gottsch (2002)
Invest. Ophthalmol. Vis. Sci. 43, 2677-2684
   Abstract »    Full Text »    PDF »
Receptor for Fc on the Surfaces of Schistosomes.
A. Loukas, M. K. Jones, L. T. King, P. J. Brindley, and D. P. McManus (2001)
Infect. Immun. 69, 3646-3651
   Abstract »    Full Text »    PDF »
The Immunodominant Brugia malayi Paramyosin as a Marker of Current Infection with Wuchereria bancrofti Adult Worms.
S. Langy, C. Plichart, P. Luquiaud, S. A. Williams, and L. Nicolas (1998)
Infect. Immun. 66, 2854-2858
   Abstract »    Full Text »    PDF »
Immunity to schistosomes: progress toward vaccine.
A Capron, J. Dessaint, M Capron, J. Ouma, and A. Butterworth (1987)
Science 238, 1065-1072
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