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Science 19 September 1986:
Vol. 233. no. 4770, pp. 1316 - 1318
DOI: 10.1126/science.3749879
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Articles

Science, Vol 233, Issue 4770, 1316-1318
Copyright © 1986 by American Association for the Advancement of Science

articles

Static and initiator protein-enhanced bending of DNA at a replication origin

RR Koepsel and SA Khan

DNA bending has been suggested to play a role in the regulation of gene expression, initiation of DNA replication, DNA packaging, and the recognition of specific DNA sequences by proteins. It has recently been demonstrated that DNA bending can be sequence-directed. Bent DNA has also been observed as a consequence of sequence-specific binding of proteins to DNA. In this report DNA of plasmid pT181 is shown to contain a bend at the replication origin. Furthermore, this bend is enhanced by the binding of the pT181 replication initiator protein, RepC, to the origin.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Kaposi's Sarcoma-Associated Herpesvirus Latency-Associated Nuclear Antigen Induces a Strong Bend on Binding to Terminal Repeat DNA.
L.-Y. Wong and A. C. Wilson (2005)
J. Virol. 79, 13829-13836
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Centromere parC of plasmid R1 is curved.
C. Hoischen, A. Bolshoy, K. Gerdes, and S. Diekmann (2004)
Nucleic Acids Res. 32, 5907-5915
   Abstract »    Full Text »    PDF »
The global intrinsic curvature of archaeal and eubacterial genomes is mostly contained in their dinucleotide composition and is probably not an adaptation.
E. Merino and A. Garciarrubio (2000)
Nucleic Acids Res. 28, 2431-2438
   Abstract »    Full Text »    PDF »
Role of Individual Monomers of a Dimeric Initiator Protein in the Initiation and Termination of Plasmid Rolling Circle Replication.
T.-L. Chang, M. G. Kramer, R. A. Ansari, and S. A. Khan (2000)
J. Biol. Chem. 275, 13529-13534
   Abstract »    Full Text »    PDF »
An Oligonucleotide Inhibits Oligomerization of a Rolling Circle Initiator Protein at the pT181 Origin of Replication.
A. C. Zhao, R. A. Ansari, M. C. Schmidt, and S. A. Khan (1998)
J. Biol. Chem. 273, 16082-16089
   Abstract »    Full Text »    PDF »
Replication and Control of Circular Bacterial Plasmids.
G. del Solar, R. Giraldo, M. J. Ruiz-Echevarria, M. Espinosa, and R. Diaz-Orejas (1998)
Microbiol. Mol. Biol. Rev. 62, 434-464
   Abstract »    Full Text »    PDF »
Replication Origin of the Broad Host Range Plasmid RK2. POSITIONING OF VARIOUS MOTIFS IS CRITICAL FOR INITIATION OF REPLICATION.
K. S. Doran, I. Konieczny, and D. R. Helinski (1998)
J. Biol. Chem. 273, 8447-8453
   Abstract »    Full Text »    PDF »
The Inactive pT181 Initiator Heterodimer, RepC/C, Binds but Fails to Induce Melting of the Plasmid Replication Origin.
R. Jin, X. Zhou, and R. P. Novick (1996)
J. Biol. Chem. 271, 31086-31091
   Abstract »    Full Text »    PDF »
DNA bending by negative regulatory proteins: Gal and Lac repressors..
C Zwieb, J Kim, and S Adhya (1989)
Genes & Dev. 3, 606-611
   Abstract »    PDF »
Direct evidence for DNA bending at the lambda replication origin.
K Zahn and F. Blattner (1987)
Science 236, 416-422
   Abstract »    PDF »
Specific Binding of Single-stranded Telomeric DNA by Cdc13p of Saccharomyces cerevisiae.
Y.-C. Lin, C.-L. Hsu, J.-W. Shih, and J.-J. Lin (2001)
J. Biol. Chem. 276, 24588-24593
   Abstract »    Full Text »    PDF »


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