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Science 29 August 1986:
Vol. 233. no. 4767, pp. 980 - 984
DOI: 10.1126/science.3016899
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Articles

Science, Vol 233, Issue 4767, 980-984
Copyright © 1986 by American Association for the Advancement of Science

articles

Immortalization of human T lymphocytes after transfection of Epstein-Barr virus DNA

M Stevenson, B Volsky, M Hedenskog, and DJ Volsky

Epstein-Barr virus (EBV), a ubiquitous human herpesvirus, has the ability to transform human B lymphocytes. No other cell type has been experimentally transformed by EBV, either by intact virions or naked viral DNA and subgenomic fragments. Two immortalized human T-lymphoblastoid cell lines have now been established by transfecting cord blood lymphocytes with purified B95-8 viral DNA enclosed in fusogenic Sendai virus envelopes (RSVE) and then exposing the cells to EBV from a P3HR-1 cell subclone. One of these lines, which has been fully characterized, is termed HBD-1. This line is positive for EBV DNA and expresses surface OKT11, OKT4, and Tac receptors, but not M-1, mu immunoglobulin chains, EBV receptors, or B-1 surface markers. The cells contain fully rearranged T-cell receptor genes and germline immunoglobulin genes. The karyotype of the cells is normal, they do not require interleukin-2 for growth, and do not contain human T-lymphotropic virus type I. However, the HBD-1 cells contain incomplete EBV genomes and express several EBV-determined antigens, including the early antigen type D, membrane antigens, but not EBV-determined nuclear antigen (EBNA). This association of the EBV genome with permanently growing hematopoietic cells of non B-cell lineage should prove useful in studies on the mechanism of EBV-mediated cell transformation.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
A Syndrome of Peripheral Blood T-Cell Infection With Epstein-Barr Virus (EBV) Followed by EBV-Positive T-Cell Lymphoma.
H. Kanegane, K. Bhatia, M. Gutierrez, H. Kaneda, T. Wada, A. Yachie, H. Seki, T. Arai, S.-i. Kagimoto, M. Okazaki, et al. (1998)
Blood 91, 2085-2091
   Abstract »    Full Text »    PDF »
Isolation and Characterization of Transformed Human T-Cell Lines Infected by Epstein-Barr Virus.
H. Groux, F. Cottrez, C. Montpellier, B. Quatannens, J. Coll, D. Stehelin, and C. Auriault (1997)
Blood 89, 4521-4530
   Abstract »    Full Text »    PDF »


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