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Science 15 August 1986:
Vol. 233. no. 4765, pp. 776 - 778
DOI: 10.1126/science.2874611
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Articles

Science, Vol 233, Issue 4765, 776-778
Copyright © 1986 by American Association for the Advancement of Science

articles

Transplantation of fetal hematopoietic stem cells in utero: the creation of hematopoietic chimeras

AW Flake, MR Harrison, NS Adzick, and ED Zanjani

Transplantation of normal, immature, fetal hematopoietic cells into a preimmune fetal recipient with a congenital hemoglobinopathy may allow partial reconstitution of normal hemoglobin production without the complications associated with postnatal bone marrow transplantation (immunosuppression and the occurrence of graft versus host disease). In order to test this hypothesis the naturally occurring polymorphism at the beta-hemoglobin locus of the sheep was used as a marker for engraftment and hematopoietic chimerism. Intraperitoneal injection of allogeneic fetal stem cells into normal fetal lambs resulted in hematopoietic chimerism in three of four surviving recipients. This chimerism has been sustained for 6 months after birth and 9 months after engraftment, without evidence of graft versus host disease, and without the use of immunosuppressive therapy.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
A New Human Somatic Stem Cell from Placental Cord Blood with Intrinsic Pluripotent Differentiation Potential.
G. Kogler, S. Sensken, J. A. Airey, T. Trapp, M. Muschen, N. Feldhahn, S. Liedtke, R. V. Sorg, J. Fischer, C. Rosenbaum, et al. (2004)
J. Exp. Med. 200, 123-135
   Abstract »    Full Text »    PDF »
High-level allogeneic chimerism achieved by prenatal tolerance induction and postnatal nonmyeloablative bone marrow transplantation.
W. H. Peranteau, S. Hayashi, M. Hsieh, A. F. Shaaban, and A. W. Flake (2002)
Blood 100, 2225-2234
   Abstract »    Full Text »    PDF »
Complete allogeneic hematopoietic chimerism achieved by a combined strategy of in utero hematopoietic stem cell transplantation and postnatal donor lymphocyte infusion.
S. Hayashi, W. H. Peranteau, A. F. Shaaban, and A. W. Flake (2002)
Blood 100, 804-812
   Abstract »    Full Text »    PDF »
In utero fetal liver hematopoietic stem cell transplantation: is there a role for alloreactive T lymphocytes?.
M. C. Renda, E. Fecarotta, A. Maggio, F. Dieli, G. Sireci, A. Salerno, L. Markling, M. Westgren, G. Damiani, C. Jakil, et al. (2000)
Blood 96, 1608-1609
   Full Text »    PDF »
In Utero Hematopoietic Stem Cell Transplantation: Ontogenic Opportunities and Biologic Barriers.
A. W. Flake and E. D. Zanjani (1999)
Blood 94, 2179-2191
   Full Text »    PDF »
Homing of Human Cells in the Fetal Sheep Model: Modulation by Antibodies Activating or Inhibiting Very Late Activation Antigen-4-Dependent Function.
E. D. Zanjani, A. W. Flake, G. Almeida-Porada, N. Tran, and T. Papayannopoulou (1999)
Blood 94, 2515-2522
   Abstract »    Full Text »    PDF »
AC133, a Novel Marker for Human Hematopoietic Stem and Progenitor Cells.
A. H. Yin, S. Miraglia, E. D. Zanjani, G. Almeida-Porada, M. Ogawa, A. G. Leary, J. Olweus, J. Kearney, and D. W. Buck (1997)
Blood 90, 5002-5012
   Abstract »    Full Text »    PDF »
In Utero Hematopoietic Stem Cell Transplantation: A Status Report.
A. W. Flake and E. D. Zanjani (1997)
JAMA 278, 932-937
   Abstract »    PDF »
Treatment of X-Linked Severe Combined Immunodeficiency by in Utero Transplantation of Paternal Bone Marrow.
A. W. Flake, M.-G. Roncarolo, J. M. Puck, G. Almeida-Porada, M. I. Evans, M. P. Johnson, E. M. Abella, D. D. Harrison, and E. D. Zanjani (1996)
N. Engl. J. Med. 335, 1806-1810
   Full Text »    PDF »
In Utero Stem Cell Transplantation.
C. Drake (1994)
Journal of Diagnostic Medical Sonography 10, 201-206
   Abstract »    PDF »


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