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Science 6 June 1986:
Vol. 232. no. 4755, pp. 1253 - 1255
DOI: 10.1126/science.232.4755.1253
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Articles

Cultivation of the Drosophila Sex-Ratio Spiroplasma

KEVIN J. HACKETT 1, DWIGHT E. LYNN 1, DAVID L. WILLIAMSON 2, ANNETTE S. GINSBERG 1, and ROBERT F. WHITCOMB 1

1 Insect Pathology Laboratory, U. S. Department of Agriculture, Beltsville, MD 20705.
2 Department of Anatomical Sciences, State University of New York, Stony Brook, NY 11794.

Uncultivable for more than 25 years, the sex-ratio spiroplasma of Drosophila willistoni grew in a tissue culture medium (H-2) containing an embryo-derived lepidopteran cell line (IPLB-TN-R2). After adaptation, it grew in a cell-free H-2 medium. This success demonstrates the usefulness of cell culture systems for cultivation of fastidious microorganisms and facilitates study of the sex-ratio trait in Drosophila.

Submitted on December 13, 1985
Accepted on March 27, 1986


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