Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Science 30 May 1986:
Vol. 232. no. 4754, pp. 1121 - 1123
DOI: 10.1126/science.3704638
Prev | Table of Contents | Next

Articles

Science, Vol 232, Issue 4754, 1121-1123
Copyright © 1986 by American Association for the Advancement of Science

articles

Relation between work and phosphate metabolite in the in vivo paced mammalian heart

RS Balaban, HL Kantor, LA Katz, and RW Briggs

Nuclear magnetic resonance (NMR) spectroscopy was used to monitor, on a beat-to-beat basis, the concentration of creatine phosphate and adenosine triphosphate during alterations in the work output of canine hearts in vivo. Over a wide range of rate-pressure products (5,000 to 25,000 mmHg/min), the relative amounts of creatine phosphate and adenosine triphosphate within the heart remained constant. The relative concentration of free adenosine diphosphate was calculated under the reasonable assumption that the creatine kinase-catalyzed reaction is near equilibrium in this tissue. The free concentration of adenosine diphosphate also did not change over this range of rate-pressure products. The data demonstrate that the concentration of these compounds is highly regulated in vivo and suggest that factors other than their concentration may be involved in the modulation of steady-state myocardial work output with oxygen consumption.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Magnitude and control of mitochondrial sensitivity to ADP.
J. A. L. Jeneson, J. P. J. Schmitz, N. M. A. van den Broek, N. A. W. van Riel, P. A. J. Hilbers, K. Nicolay, and J. J. Prompers (2009)
Am J Physiol Endocrinol Metab 297, E774-E784
   Abstract »    Full Text »    PDF »
Phosphate metabolite concentrations and ATP hydrolysis potential in normal and ischaemic hearts.
F. Wu, E. Y. Zhang, J. Zhang, R. J. Bache, and D. A. Beard (2008)
J. Physiol. 586, 4193-4208
   Abstract »    Full Text »    PDF »
Modular regulation analysis of heart contraction: application to in situ demonstration of a direct mitochondrial activation by calcium in beating heart.
P. Diolez, V. Deschodt-Arsac, G. Raffard, C. Simon, P. D. Santos, E. Thiaudiere, L. Arsac, and J.-M. Franconi (2007)
Am J Physiol Regulatory Integrative Comp Physiol 293, R13-R19
   Abstract »    Full Text »    PDF »
Parallel activation of mitochondrial oxidative metabolism with increased cardiac energy expenditure is not dependent on fatty acid oxidation in pigs.
L. Zhou, M. E. Cabrera, H. Huang, C. L. Yuan, D. K. Monika, N. Sharma, F. Bian, and W. C. Stanley (2007)
J. Physiol. 579, 811-821
   Abstract »    Full Text »    PDF »
Altered high-energy phosphate metabolism predicts contractile dysfunction and subsequent ventricular remodeling in pressure-overload hypertrophy mice.
M. Y. Maslov, V. P. Chacko, M. Stuber, A. L. Moens, D. A. Kass, H. C. Champion, and R. G. Weiss (2007)
Am J Physiol Heart Circ Physiol 292, H387-H391
   Abstract »    Full Text »    PDF »
Molecular system bioenergetics: regulation of substrate supply in response to heart energy demands.
V. Saks, R. Favier, R. Guzun, U. Schlattner, and T. Wallimann (2006)
J. Physiol. 577, 769-777
   Abstract »    Full Text »    PDF »
Metabolic control over the oxygen consumption flux in intact skeletal muscle: in silico studies.
P. Liguzinski and B. Korzeniewski (2006)
Am J Physiol Cell Physiol 291, C1213-C1224
   Abstract »    Full Text »    PDF »
Regulation of myocardial substrate metabolism during increased energy expenditure: insights from computational studies.
L. Zhou, M. E. Cabrera, I. C. Okere, N. Sharma, and W. C. Stanley (2006)
Am J Physiol Heart Circ Physiol 291, H1036-H1046
   Abstract »    Full Text »    PDF »
Oxygen consumption and metabolite concentrations during transitions between different work intensities in heart.
B. Korzeniewski (2006)
Am J Physiol Heart Circ Physiol 291, H1466-H1474
   Abstract »    Full Text »    PDF »
Myocardial Substrate Metabolism in the Normal and Failing Heart.
W. C. Stanley, F. A. Recchia, and G. D. Lopaschuk (2005)
Physiol Rev 85, 1093-1129
   Abstract »    Full Text »    PDF »
Control of respiration and bioenergetics during muscle contraction.
Y. Chung, P. A. Mole, N. Sailasuta, T. K. Tran, R. Hurd, and T. Jue (2005)
Am J Physiol Cell Physiol 288, C730-C738
   Abstract »    Full Text »    PDF »
Regulation of pyruvate dehydrogenase activity and citric acid cycle intermediates during high cardiac power generation.
N. Sharma, I. C. Okere, D. Z. Brunengraber, T. A. McElfresh, K. L. King, J. P. Sterk, H. Huang, M. P. Chandler, and W. C. Stanley (2005)
J. Physiol. 562, 593-603
   Abstract »    Full Text »    PDF »
Is the Failing Heart Energy Starved?: On Using Chemical Energy to Support Cardiac Function.
J. S. Ingwall and R. G. Weiss (2004)
Circ. Res. 95, 135-145
   Abstract »    Full Text »    PDF »
Myocardial Energy Metabolism During Ischemia and the Mechanisms of Metabolic Therapies.
W. C. Stanley (2004)
Journal of Cardiovascular Pharmacology and Therapeutics 9, S31-S45
   Abstract »    PDF »
Interstitial purine metabolites in hearts with LV remodeling.
A. V. Gourine, Q. Hu, P. R. Sander, A. I. Kuzmin, N. Hanafy, S. A. Davydova, D. V. Zaretsky, and J. Zhang (2004)
Am J Physiol Heart Circ Physiol 286, H677-H684
   Abstract »    Full Text »    PDF »
Regulation of murine myocardial energy metabolism during adrenergic stress studied by in vivo 31P NMR spectroscopy.
A. V. Naumova, R. G. Weiss, and V. P. Chacko (2003)
Am J Physiol Heart Circ Physiol 285, H1976-H1979
   Abstract »    Full Text »    PDF »
Metabolic Network Control of Oxidative Phosphorylation: MULTIPLE ROLES OF INORGANIC PHOSPHATE.
S. Bose, S. French, F. J. Evans, F. Joubert, and R. S. Balaban (2003)
J. Biol. Chem. 278, 39155-39165
   Abstract »    Full Text »    PDF »
Metabolic consequences of functional complexes of mitochondria, myofibrils and sarcoplasmic reticulum in muscle cells.
T. Andrienko, A. V. Kuznetsov, T. Kaambre, Y. Usson, A. Orosco, F. Appaix, T. Tiivel, P. Sikk, M. Vendelin, R. Margreiter, et al. (2003)
J. Exp. Biol. 206, 2059-2072
   Abstract »    Full Text »    PDF »
Role of calcium in metabolic signaling between cardiac sarcoplasmic reticulum and mitochondria in vitro.
R. S. Balaban, S. Bose, S. A. French, and P. R. Territo (2003)
Am J Physiol Cell Physiol 284, C285-C293
   Abstract »    Full Text »    PDF »
Absolute concentrations of high-energy phosphate metabolites in normal, hypertrophied, and failing human myocardium measured noninvasively with 31P-SLOOP magnetic resonance spectroscopy.
M. Beer, T. Seyfarth, J.o. Sandstede, W. Landschutz, C. Lipke, H. Kostler, M. von Kienlin, K. Harre, D. Hahn, and S. Neubauer (2002)
J. Am. Coll. Cardiol. 40, 1267-1274
   Abstract »    Full Text »    PDF »
Activation time of myocardial oxidative phosphorylation in creatine kinase and adenylate kinase knockout mice.
L. A. Gustafson and J. H. G. M. Van Beek (2002)
Am J Physiol Heart Circ Physiol 282, H2259-H2264
   Abstract »    Full Text »    PDF »
Changes in cardiac metabolism: a critical step from stable angina to ischaemic cardiomyopathy.
W.C. Stanley (2001)
Eur. Heart J. Suppl. 3, O2-O7
   Abstract »    PDF »
Effects of augmented delivery of pyruvate on myocardial high-energy phosphate metabolism at high workstate.
K. Ochiai, J. Zhang, G. Gong, Y. Zhang, J. Liu, Y. Ye, X. Wu, H. Liu, Y. Murakami, R. J. Bache, et al. (2001)
Am J Physiol Heart Circ Physiol 281, H1823-H1832
   Abstract »    Full Text »    PDF »
Mitochondrial ATPase and high-energy phosphates in failing hearts.
J. Liu, C. Wang, Y. Murakami, G. Gong, Y. Ishibashi, C. Prody, K. Ochiai, R. J. Bache, C. Godinot, and J. Zhang (2001)
Am J Physiol Heart Circ Physiol 281, H1319-H1326
   Abstract »    Full Text »    PDF »
Oxygen supply and oxidative phosphorylation limitation in rat myocardium in situ.
U. Kreutzer, Y. Mekhamer, Y. Chung, and T. Jue (2001)
Am J Physiol Heart Circ Physiol 280, H2030-H2037
   Abstract »    Full Text »    PDF »
MRI/MRS assessment of in vivo murine cardiac metabolism, morphology, and function at physiological heart rates.
V. P. Chacko, F. Aresta, S. M. Chacko, and R. G. Weiss (2000)
Am J Physiol Heart Circ Physiol 279, H2218-H2224
   Abstract »    Full Text »    PDF »
Regulation of mitochondrial respiration in heart cells analyzed by reaction-diffusion model of energy transfer.
M. Vendelin, O. Kongas, and V. Saks (2000)
Am J Physiol Cell Physiol 278, C747-C764
   Abstract »    Full Text »    PDF »
Chest Pain in Women with Normal Coronary Angiograms.
R. O. Cannon and R. S. Balaban (2000)
N. Engl. J. Med. 342, 885-887
   Full Text »
Direct Evidence for the Control of Mitochondrial Respiration by Mitochondrial Creatine Kinase in Oxidative Muscle Cells in Situ.
L. Kay, K. Nicolay, B. Wieringa, V. Saks, and T. Wallimann (2000)
J. Biol. Chem. 275, 6937-6944
   Abstract »    Full Text »    PDF »
Free [ADP] and Aerobic Muscle Work Follow at Least Second Order Kinetics in Rat Gastrocnemius in Vivo.
J. H. Cieslar and G. P. Dobson (2000)
J. Biol. Chem. 275, 6129-6134
   Abstract »    Full Text »    PDF »
Ca2+ activation of heart mitochondrial oxidative phosphorylation: role of the F0/F1-ATPase.
P. R. Territo, V. K. Mootha, S. A. French, and R. S. Balaban (2000)
Am J Physiol Cell Physiol 278, C423-C435
   Abstract »    Full Text »    PDF »
Overexpression of the Cardiac {beta}2-Adrenergic Receptor and Expression of a {beta}-Adrenergic Receptor Kinase-1 ({beta}ARK1) Inhibitor Both Increase Myocardial Contractility but Have Differential Effects on Susceptibility to Ischemic Injury.
H. R. Cross, C. Steenbergen, R. J. Lefkowitz, W. J. Koch, and E. Murphy (1999)
Circ. Res. 85, 1077-1084
   Abstract »    Full Text »    PDF »
Dynamic adaptation of cardiac oxidative phosphorylation is not mediated by simple feedback control.
J. H. G. M. van Beek, M. H. van Wijhe, M. H. J. Eijgelshoven, and J. B. Hak (1999)
Am J Physiol Heart Circ Physiol 277, H1375-H1384
   Abstract »    Full Text »    PDF »
Regulation of respiration in myocardium in the transient and steady state.
Y. Chung and T. Jue (1999)
Am J Physiol Heart Circ Physiol 277, H1410-H1417
   Abstract »    Full Text »    PDF »
Oxygen delivery does not limit cardiac performance during high work states.
J. Zhang, Y. Murakami, Y. Zhang, Y. K Cho, Y. Ye, G. Gong, R. J. Bache, and A. H. L. From (1999)
Am J Physiol Heart Circ Physiol 277, H50-H57
   Abstract »    Full Text »    PDF »
31P NMR studies of creatine kinase flux in M-creatine kinase-deficient mouse heart.
F. A. Van Dorsten, M. G. J. Nederhoff, K. Nicolay, and C. J. A. Van Echteld (1998)
Am J Physiol Heart Circ Physiol 275, H1191-H1199
   Abstract »    Full Text »    PDF »
Inorganic phosphate content and free energy change of ATP hydrolysis in regional short-term hibernating myocardium.
C. Martin, R. Schulz, J. Rose, and G. Heusch (1998)
Cardiovasc Res 39, 318-326
   Abstract »    Full Text »    PDF »
The beta /alpha Peak Height Ratio of ATP. A MEASURE OF FREE [Mg2+] USING 31P NMR.
K. Clarke, Y. Kashiwaya, M. T. King, D. Gates, C. A. Keon, H. R. Cross, G. K. Radda, and R. L. Veech (1996)
J. Biol. Chem. 271, 21142-21150
   Abstract »    Full Text »    PDF »
Myocardial High-Energy Phosphate and Substrate Metabolism in Swine With Moderate Left Ventricular Hypertrophy.
B. M. Massie, S. Schaefer, J. Garcia, M. D. McKirnan, G. G. Schwartz, J. A. Wisneski, M. W. Weiner, and F. C. White (1995)
Circulation 91, 1814-1823
   Abstract »    Full Text »
Sodium Pentobarbital Versus {alpha}-Chloralose Anesthesia : Experimental Production of Substantially Different Slopes in the Transmural CP/ATP Ratios Within the Left Ventricle of the Canine Myocardium.
D. P. Rath, C. M. Little, H. Zhang, Z. Jiang, A. M. Abduljalil, H. Zhu, X. Tong, C. Brown, R. L. Hamlin, and P.-M. L. Robitaille (1995)
Circulation 91, 471-475
   Abstract »    Full Text »
Calcium Activation of Heart Mitochondrial Oxidative Phosphorylation. RAPID KINETICS OF mVO2, NADH, AND LIGHT SCATTERING.
P. R. Territo, S. A. French, M. C. Dunleavy, F. J. Evans, and R. S. Balaban (2001)
J. Biol. Chem. 276, 2586-2599
   Abstract »    Full Text »    PDF »
Activation time of myocardial oxidative phosphorylation in creatine kinase and adenylate kinase knockout mice.
L. A. Gustafson and J. H. G. M. Van Beek (2002)
Am J Physiol Heart Circ Physiol 282, H2259-H2264
   Abstract »    Full Text »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)