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Science 28 March 1986:
Vol. 231. no. 4745, pp. 1564 - 1566
DOI: 10.1126/science.2420007
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Articles

Science, Vol 231, Issue 4745, 1564-1566
Copyright © 1986 by American Association for the Advancement of Science

articles

Calcium channels in planar lipid bilayers: insights into mechanisms of ion permeation and gating

RL Rosenberg, P Hess, JP Reeves, H Smilowitz, and RW Tsien

Electrophysiological recordings were used to analyze single calcium channels in planar lipid bilayers after membranes from bovine cardiac sarcolemmal vesicles had been incorporated into the bilayer. In these cell-free conditions, channels in the bilayer showed unitary barium or calcium conductances, gating kinetics, and pharmacological responses that were similar to dihydropyridine-sensitive calcium channels in intact cells. The open channel current varied in a nonlinear manner with voltage under asymmetric (that is, physiological) ionic conditions. However, with identical solutions on both sides of the bilayer, the current-voltage relation was linear. In matched experiments, calcium channels from skeletal muscle T-tubules differed significantly from cardiac calcium channels in their conductance properties and gating kinetics.


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