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Science 14 March 1986:
Vol. 231. no. 4743, pp. 1289 - 1291
DOI: 10.1126/science.2418504
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Articles

Science, Vol 231, Issue 4743, 1289-1291
Copyright © 1986 by American Association for the Advancement of Science

articles

Characterization of highly immunogenic p66/p51 as the reverse transcriptase of HTLV-III/LAV

F di Marzo Veronese, TD Copeland, AL DeVico, R Rahman, S Oroszlan, RC Gallo, and MG Sarngadharan

Approximately 80 percent of all human sera that react with antigens of HTLV-III, the etiologic agent of the acquired immune deficiency syndrome (AIDS), recognize protein bands at 66 and 51 kilodaltons. A mouse hybridoma was produced that was specific to these proteins. Repeated cloning of the hybridoma did not separate the two reactivities. The p66/p51 was purified from HTLV-III lysates by immunoaffinity chromatography and subjected to NH2-terminal Edman degradation. Single amino acid residues were obtained in 17 successive degradation cycles. The sequence determined was a perfect translation of the nucleotide sequence of a portion of the HTLV-III pol gene. The purified p66/51 had reverse transcriptase activity and the monoclonal immunoglobulin G specifically removed the enzyme activity from crude viral extract as well as purified enzyme.


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