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Science 14 March 1986:
Vol. 231. no. 4743, pp. 1287 - 1289
DOI: 10.1126/science.231.4743.1287
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Articles

Monooxygenase Induction and Chlorobiphenyls in the Deep-Sea Fish Coryphaenoides armatus

JOHN J. STEGEMAN 1, PAMELA J. KLOEPPER-SAMS 1, and JOHN W. FARRINGTON 2

1 Department of Biology, Woods Hole Oceanographic Institution, Woods Hole, MA 02543.
2 Department of Chemistry and Coastal Research Center, Woods Hole Oceanographic Institution, Woods Hole, MA 02543.

Inhibition of liver microsomal ethoxyresorufin O-deethylase and aryl hydrocarbon hydroxylase activities by agr-naphthoflavone and by polyclonal antibodies to hydrocarbon-inducible cytochrome P-450E from teleost liver indicated a xenobiotic-induced origin of these activities in the deep-sea fish Coryphaenoides armatus. Specific recognition of a protein by antibodies to P-450E in an immunoblot assay further indicated xenobiotic-induced cytochrome P-450 in these animals. Levels of apparently induced cytochrome P-450 and monooxygenase activity correlated positively with the tissue content of chlorobiphenyls of known inducing activity, implicating such compounds in biochemical effects occurring in the deep ocean.

Submitted on August 19, 1985
Accepted on December 20, 1985

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Science. ISSN 0036-8075 (print), 1095-9203 (online)