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Science 21 February 1986:
Vol. 231. no. 4740, pp. 850 - 853
DOI: 10.1126/science.2418502
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Articles

Science, Vol 231, Issue 4740, 850-853
Copyright © 1986 by American Association for the Advancement of Science

articles

Long-term cultures of HTLV-III--infected T cells: a model of cytopathology of T-cell depletion in AIDS

D Zagury, J Bernard, R Leonard, R Cheynier, M Feldman, PS Sarin, and RC Gallo

Long-term cultures were established of HTLV-III-infected T4 cells from patients with the acquired immune deficiency syndrome (AIDS) and of T4 cells from normal donors after infection of the cells in vitro. By initially reducing the number of cells per milliliter of culture medium it was possible to grow the infected cells for 50 to 60 days. As with uninfected T cells, immunologic activation of the HTLV-III-infected cells with phytohemagglutinin led to patterns of gene expression typical of T-cell differentiation, such as production of interleukin-2 and expression of interleukin-2 receptors, but in the infected cells immunologic activation also led to expression of HTLV-III, which was followed by cell death. The results revealed a cytopathogenic mechanism that may account for T4 cell depletion in AIDS patients and suggest how repeated antigenic stimulation by infectious agents, such as malaria in Africa, or by allogeneic blood or semen, may be important determinants of the latency period in AIDS.


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