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Science 14 February 1986:
Vol. 231. no. 4739, pp. 725 - 727
DOI: 10.1126/science.231.4739.725
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Articles

Transfer of Agrobacterium DNA to Plants Requires a T-DNA Border But Not the virE Locus

RICHARD C. GARDNER 1 and VIC C. KNAUF 1

1 Calgene, Inc., 1920 Fifth Street, Davis, CA 95616.

Agrobacterium tumefaciens induces tumors in plants by transferring and integrating oncogenes (T-DNA) into the chromosomes of host plant cells. Agrobacterium strains were used to transfer complementary DNA copies of a potato spindle tuber viroid (PSTV) to plant cells at a wound site on tomato plant stems. Subsequently, infectious viroid RNA was found in the leaves of these plants, indicating systemic PSTV infection. This process utilized the T-DNA transfer mechanisms of Agrobacterium since PSTV infection required most virulence genes (vir) as well as one of the DNA sequences that flank either side of the Agrobacterium T-DNA. However, transfer still occurred from virE mutants of Agrobacterium, strains that fail to induce tumors even though a completely functional T-DNA is present. The virE gene seems to be directly involved in the integration of foreign DNA into plant chromosomes.

Submitted on May 16, 1985
Accepted on November 5, 1985


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Analysis of Vir protein translocation from Agrobacterium tumefaciens using Saccharomyces cerevisiae as a model: evidence for transport of a novel effector protein VirE3.
B. Schrammeijer, A. d. Dulk-Ras, A. C. Vergunst, E. Jurado Jacome, and P. J. J. Hooykaas (2003)
Nucleic Acids Res. 31, 860-868
   Abstract »    Full Text »    PDF »
Interaction of the DNA modifying proteins VirD1 and VirD2 of Agrobacterium tumefaciens: Analysis by subcellular localization in mammalian cells.
B. Relić, L. Rossi, Y. Nagamine, and B. Hohn (1998)
PNAS 95, 9105-9110
   Abstract »    Full Text »    PDF »
Single-Stranded DNA Binding Protein Encoded by the virE Locus of Agrobacterium tumefaciens.
V. CITOVSKY, G. DE VOS, and P. ZAMBRYSKI (1988)
Science 240, 501-504
   Abstract »    PDF »


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