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Science 7 February 1986:
Vol. 231. no. 4738, pp. 616 - 618
DOI: 10.1126/science.3511528
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Articles

Science, Vol 231, Issue 4738, 616-618
Copyright © 1986 by American Association for the Advancement of Science

articles

Molecular basis for the auxin-independent phenotype of crown gall tumor tissues

MF Thomashow, S Hugly, WG Buchholz, and LS Thomashow

The transfer of specific Ti (tumor-inducing) plasmid sequences, the T-DNA, from Agrobacterium tumefaciens to a wide range of plants results in the formation of crown gall tumors. These tissues differ from most plant cells in that they can be grown in vitro in the absence of added phytohormones. Here, data are presented that offer an explanation for the auxin-independent phenotype of crown gall tissues. It is shown that crude cell-free extracts prepared from three bacterial species harboring pTiA6 gene 1 could convert L-tryptophan to indole-3-acetamide; control extracts lacking gene 1 could not carry out the reaction. Other reports indicate that the pTiA6 gene 2 product can convert indole-3-acetamide to indole-3-acetic acid, a naturally occurring auxin of plants. It is concluded that the auxin-independent phenotype of crown gall tissue involves the introduction of Ti plasmid sequences encoding a two-step pathway for auxin synthesis.


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