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Science 10 January 1986:
Vol. 231. no. 4734, pp. 134 - 139
DOI: 10.1126/science.3079915
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Articles

Science, Vol 231, Issue 4734, 134-139
Copyright © 1986 by American Association for the Advancement of Science

articles

Automated chemical synthesis of a protein growth factor for hemopoietic cells, interleukin-3

I Clark-Lewis, R Aebersold, H Ziltener, JW Schrader, LE Hood, and SB Kent

Interleukin-3 (IL-3), a protein of 140 amino acids, was chemically synthesized by means of an automated peptide synthesizer and was shown to have the biological activities attributed to native IL-3. Assays of synthetic analogues established that an amino terminal fragment has detectable IL-3 activity, but that the stable tertiary structure of the complete molecule was required for full activity. The results demonstrate that automated peptide synthesis can be applied to the study of the structure and function of proteins.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Protection Against Experimental Autoimmune Encephalomyelitis Generated by a Recombinant Adenovirus Vector Expressing the V{beta}8.2 TCR Is Disrupted by Coadministration with Vectors Expressing Either IL-4 or -10.
T. A. Braciak, B. Pedersen, J. Chin, C. Hsiao, E. S. Ward, I. Maricic, A. Jahng, F. L. Graham, J. Gauldie, E. E. Sercarz, et al. (2003)
J. Immunol. 170, 765-774
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Structural Studies of a Peptide Activator of Human Lecithin-Cholesterol Acyltransferase.
G. W. Buchko, W. D. Treleaven, S. J. Dunne, A. S. Tracey, and R. J. Cushley (1996)
J. Biol. Chem. 271, 3039-3045
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Peptide and protein synthesis by segment synthesis-condensation.
E. Kaiser, H Mihara, G. Laforet, J. Kelly, L Walters, M. Findeis, and T Sasaki (1989)
Science 243, 187-192
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Epitopes recognized by human T cells map within the conserved part of the GP190 of P. falciparum.
A Crisanti, H. Muller, C Hilbich, F Sinigaglia, H Matile, M McKay, J Scaife, K Beyreuther, and H Bujard (1988)
Science 240, 1324-1326
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Biotechnology and Medicine of the Future.
L. Hood (1988)
JAMA 259, 1837-1844
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Solid phase synthesis.
B Merrifield (1986)
Science 232, 341-347
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