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Science 20 December 1985:
Vol. 230. no. 4732, pp. 1383 - 1385
DOI: 10.1126/science.3906896
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Articles

Science, Vol 230, Issue 4732, 1383-1385
Copyright © 1985 by American Association for the Advancement of Science

articles

Systematic variation in DNA length yields highly ordered repressor-operator cocrystals

Jordan SR, TV Whitcombe, JM Berg, and CO Pabo

Crystals have been grown that contain the operator-binding domain of the lambda repressor and the lambda operator site OL1. Crystallization conditions were tested with a set of DNA fragments, ranging in length from 17 to 23 base pairs. The best crystals were grown with a 20-base pair DNA fragment. These crystals have space-group symmetry P2I, with unit cell dimensions a = 37.1 A, b = 68.8 A, c = 56.8 A, and a beta angle of 91.5 degrees. They diffracted to at least 2.5 A resolution. High resolution data from these crystals should allow the direct determination of how a repressor recognizes its operator site.


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