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Science 6 December 1985:
Vol. 230. no. 4730, pp. 1160 - 1163
DOI: 10.1126/science.3865370
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Articles

Science, Vol 230, Issue 4730, 1160-1163
Copyright © 1985 by American Association for the Advancement of Science

articles

Specific expression of hepatitis B surface antigen (HBsAg) in transgenic mice

C Babinet, H Farza, D Morello, M Hadchouel, and C Pourcel

Two transgenic mice were obtained that contain in their chromosomes the complete hepatitis B virus (HBV) genome except for the core gene. These mice secrete particles of HBV surface antigen (HBsAg) in the serum. In one mouse, HBV DNA sequences that had integrated at two different sites were shown to segregate independently in the first filial generation (F1) and only one of the sequences allowed expression of the surface antigen. Among these animals the males produced five to ten times more HBsAg than the females. A 2.1-kilobase messenger RNA species comigrating with the major surface gene messenger RNA is expressed specifically in the liver in the two original mice. The results suggest that the HBV sequences introduced into the mice are able to confer a tissue-specific expression to the S gene. In addition, the HBV transgenic mice represent a new model for the chronic carrier state of hepatitis B virus infection.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
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J. Virol. 79, 13116-13128
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HBsAg/HLA-A2 transgenic mice: a model for T cell tolerance to hepatitis B surface antigen in chronic hepatitis B virus infection.
D. Loirat, M. Mancini-Bourgine, J.-P. Abastado, and M.-L. Michel (2003)
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CpG Oligodeoxynucleotides with Hepatitis B Surface Antigen (HBsAg) for Vaccination in HBsAg-Transgenic Mice.
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J. Virol. 75, 6482-6491
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A Dominant Modifier of Transgene Methylation Is Mapped by QTL Analysis to Mouse Chromosome 13.
P. Valenza-Schaerly, B. Pickard, J. Walter, M. Jung, L. Pourcel, W. Reik, D. Gauguier, G. Vergnaud, and C. Pourcel (2001)
Genome Res. 11, 382-388
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The Hepatitis B Virus Core Promoter Is Strongly Activated by the Liver Nuclear Receptor Fetoprotein Transcription Factor or by Ectopically Expressed Steroidogenic Factor 1.
S. Gilbert, L. Galarneau, A. Lamontagne, S. Roy, and L. Bélanger (2000)
J. Virol. 74, 5032-5039
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Regulation of Hepatitis B Virus mRNA Expression in a Hepatitis B Surface Antigen Transgenic Mouse Model by IFN-{gamma}-Secreting T Cells After DNA-Based Immunization.
M. Mancini, M. Hadchouel, P. Tiollais, and M.-L. Michel (1998)
J. Immunol. 161, 5564-5570
   Abstract »    Full Text »    PDF »
Expression in transgenic mice of two genes of different tissue specificity integrated into a single chromosomal site..
P Einat, Y Bergman, D Yaffe, and M Shani (1987)
Genes & Dev. 1, 1075-1084
   Abstract »    PDF »


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