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Science 29 November 1985:
Vol. 230. no. 4729, pp. 1061 - 1063
DOI: 10.1126/science.4059922
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Articles

Science, Vol 230, Issue 4729, 1061-1063
Copyright © 1985 by American Association for the Advancement of Science

articles

Isolation of a mammalian sequence capable of conferring cell cycle regulation to a heterologous gene

A Artishevsky, A Grafsky, and AS Lee

A hybrid gene containing the 5' sequence of a hamster histone H3 gene and the coding sequence of the bacterial neomycin-resistance gene (neo) was constructed. Upon transfection into the hamster fibroblast cell line K12, the hybrid gene exhibited cell cycle-dependent regulation, as evidenced by the maximal accumulation of the neo transcripts during synthesis of DNA in the cell cycle. In addition, cells arrested in the prereplicative phase, as a consequence of the K12 temperature-sensitive mutation, produced significantly less neo messenger RNA.


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