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Science 15 November 1985:
Vol. 230. no. 4727, pp. 825 - 827
DOI: 10.1126/science.230.4727.825
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Articles

Cell-Assisted Growth of a Fastidious Spiroplasma

KEVIN J. HACKETT 1 and DWIGHT E. LYNN 1

1 Insect Pathology Laboratory, U.S. Department of Agriculture, Beltsville, Maryland 20705

The Colorado potato beetle spiroplasma, which is not cultivable in conventional cell-free media, grew in tissue culture media in the presence of several coleopteran and lepidopteran insect cell lines. The cultured organisms attained titers of 1.2 x 109 spiroplasmas per milliliter of culture at the 100th passage and retained infectivity and a high capacity for translational motility at the 15th passage. Cell culture systems may facilitate the isolation of other presently uncultivable microorganisms and may be useful in the study of the role of microbial physiology and behavior in pathogenicity.

Submitted on June 7, 1985
Accepted on September 18, 1985


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Biological Enrichment of Mycoplasma Agents by Cocultivation with Permissive Cell Cultures.
D. V. Volokhov, H. Kong, J. George, C. Anderson, and V. E. Chizhikov (2008)
Appl. Envir. Microbiol. 74, 5383-5391
   Abstract »    Full Text »    PDF »
Cultivation of the Drosophila Sex-Ratio Spiroplasma.
K. J. HACKETT, D. E. LYNN, D. L. WILLIAMSON, A. S. GINSBERG, and R. F. WHITCOMB (1986)
Science 232, 1253-1255
   Abstract »    PDF »


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