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Science 15 November 1985:
Vol. 230. no. 4727, pp. 813 - 814
DOI: 10.1126/science.230.4727.813
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Articles

Electrokinetic Separation of Chiral Compounds

E. GASSMANN 1, J. E. KUO 1, and R. N. ZARE 1

1 Department of Chemistry, Stanford University, Stanford, California 94305

Femtomole amounts of racemic mixtures of derivatized amino acids were resolved and analyzed rapidly in about 10 minutes by means of high-voltage zone electrophoresis with laser-fluorescence detection. The electrophoresis was performed in capillary columns containing a chiral support electrolyte. A number of dansyl amino acids were resolved by the diastereomeric interaction between the DL-amino acid and the copper(II) complex of L-histidine present in the support electrolyte. A combination of electro-osmotic and electrophoretic action caused all species, positively charged, neutral, and negatively charged, to pass through the 0.5- nanoliter detection volume where they were subjected to laser excitation.

Submitted on June 10, 1985
Accepted on August 5, 1985


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Subattomole amino acid analysis by capillary zone electrophoresis and laser-induced fluorescence.
Y. Cheng and N. Dovichi (1988)
Science 242, 562-564
   Abstract »    PDF »
Capillary Electrophoresis.
M. J. Gordon, X. Huang, S. L. Pentoney Jr., and R. N. Zare (1988)
Science 242, 224-228
   Abstract »    PDF »


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