Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Science 8 November 1985:
Vol. 230. no. 4726, pp. 663 - 666
DOI: 10.1126/science.4048951
Prev | Table of Contents | Next

Articles

Science, Vol 230, Issue 4726, 663-666
Copyright © 1985 by American Association for the Advancement of Science

articles

Intracellular free calcium localization in neutrophils during phagocytosis

DW Sawyer, JA Sullivan, and GL Mandell

Intracellular free calcium (Ca2+ i) is thought to be an important second messenger for phagocyte functions. The fluorescent indicator Quin2 was used to measure and visualize [Ca2+]i in human neutrophils during chemotaxis toward, and phagocytosis of, opsonized zymosan. In neutrophils migrating toward zymosan, [Ca2+]i was highest in the lamellipodium. Neutrophils ingesting opsonized zymosan had the highest [Ca2+]i in the pseudopods and periphagosomal cytoplasm. Most of the increase in [Ca2+]i was from extracellular sources. Regional increments in [Ca2+]i are strategically located to modulate such cellular functions as chemotaxis, oxidative activity, and degranulation.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Translocation or just location? Pseudopodia affect fluorescent signals.
S. Dewitt, R. L. Darley, and M. B. Hallett (2009)
J. Cell Biol. 184, 197-203
   Abstract »    Full Text »    PDF »
Ceramide kinase promotes Ca2+ signaling near IgG-opsonized targets and enhances phagolysosomal fusion in COS-1 cells.
V. Hinkovska-Galcheva, A. Clark, S. VanWay, J.-B. Huang, M. Hiraoka, A. Abe, M. Borofsky, R. G. Kunkel, T. Shanley, J. A. Shayman, et al. (2008)
J. Lipid Res. 49, 531-542
   Abstract »    Full Text »    PDF »
Ca2+ influx is an essential component of the positive-feedback loop that maintains leading-edge structure and activity in macrophages.
J. H. Evans and J. J. Falke (2007)
PNAS 104, 16176-16181
   Abstract »    Full Text »    PDF »
Influx of extracellular Ca2+ is necessary for electrotaxis in Dictyostelium.
L. J. Shanley, P. Walczysko, M. Bain, D. J. MacEwan, and M. Zhao (2006)
J. Cell Sci. 119, 4741-4748
   Abstract »    Full Text »    PDF »
Phagosomal oxidative activity during {beta}2 integrin (CR3)-mediated phagocytosis by neutrophils is triggered by a non-restricted Ca2+ signal: Ca2+ controls time not space.
S. Dewitt, I. Laffafian, and M. B. Hallett (2003)
J. Cell Sci. 116, 2857-2865
   Abstract »    Full Text »    PDF »
Signal transduction during Fc receptor-mediated phagocytosis.
E. Garcia-Garcia and C. Rosales (2002)
J. Leukoc. Biol. 72, 1092-1108
   Abstract »    Full Text »    PDF »
S100A12 Is Expressed Exclusively by Granulocytes and Acts Independently from MRP8 and MRP14.
T. Vogl, C. Propper, M. Hartmann, A. Strey, K. Strupat, C. van den Bos, C. Sorg, and J. Roth (1999)
J. Biol. Chem. 274, 25291-25296
   Abstract »    Full Text »    PDF »
Does cytosolic free Ca2+ signal neutrophil chemotaxis in response to formylated chemotactic peptide?.
I Laffafian and M. Hallett (1995)
J. Cell Sci. 108, 3199-3205
   Abstract »    PDF »
Calcium gradients underlying polarization and chemotaxis of eosinophils.
R. Brundage, K. Fogarty, R. Tuft, and F. Fay (1991)
Science 254, 703-706
   Abstract »    PDF »
Calcium-G Protein Interactions in the Regulation of Macrophage Secretion.
A. Di, B. Krupa, and D. J. Nelson (2001)
J. Biol. Chem. 276, 37124-37132
   Abstract »    Full Text »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)