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Science 18 October 1985:
Vol. 230. no. 4723, pp. 247 - 256
DOI: 10.1126/science.4048934
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Articles

Science, Vol 230, Issue 4723, 247-256
Copyright © 1985 by American Association for the Advancement of Science

articles

Fluorescence digital imaging microscopy in cell biology

DJ Arndt-Jovin, M Robert-Nicoud, SJ Kaufman, and TM Jovin

Developments in microscope, sensor, and image-processing technologies have led to integrated systems for the quantification of low-light-level emission signals from biological samples. Specificity is provided in the form of monoclonal antibodies and other ligands or enzyme substrates conjugated with efficient fluorophores. Fluorescent probes are also available for cellular macromolecular constituents and for free ions of biological interest such as H+ and Ca2+. The entire spectrum of photophysical phenomena can be exploited. Representative data are presented from studies of DNA conformation and architecture in polytene chromosomes and from studies of receptor-mediated endocytosis, calcium distribution, and the organization of the contractile apparatus in muscle cells.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Three-dimensional Imaging of the Intracellular Localization of Growth Hormone and Prolactin and Their mRNA Using Nanocrystal (Quantum Dot) and Confocal Laser Scanning Microscopy Techniques.
A. Matsuno, J. Itoh, S. Takekoshi, T. Nagashima, and R. Y. Osamura (2005)
J. Histochem. Cytochem. 53, 833-838
   Abstract »    Full Text »    PDF »
Fluorescence in situ hybridization: past, present and future.
J. M. Levsky and R. H. Singer (2003)
J. Cell Sci. 116, 2833-2838
   Abstract »    Full Text »    PDF »
Molecular crowding on the cell surface.
T. Ryan, J Myers, D Holowka, B Baird, and W. Webb (1988)
Science 239, 61-64
   Abstract »    PDF »


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