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Science 27 September 1985:
Vol. 229. no. 4720, pp. 1393 - 1395
DOI: 10.1126/science.2994221
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Articles

Science, Vol 229, Issue 4720, 1393-1395
Copyright © 1985 by American Association for the Advancement of Science

articles

Site-specific increased phosphorylation of pp60v-src after treatment of RSV-transformed cells with a tumor promoter

AF Purchio, M Shoyab, and LE Gentry

When vole cells that had been transformed by Rous sarcoma virus were treated with the tumor-promoting phorbol ester 12-O-tetradecanoyl-13-acetate (TPA), specific phosphorylation of pp60v-src was increased. Partial V8 protease mapping indicated that the increased phosphorylation occurred exclusively on serine residues located in the amino terminus of the molecule. Treatment of cells with dimethyl sulfoxide or 4 alpha-phorbol-12,13-didecanoate did not elicit this response. Two-dimensional tryptic phosphopeptide mapping of pp60v-src immunoprecipitated from untreated and TPA-treated cells indicated that a specific tryptic amino-terminal peptide was hyperphosphorylated.


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