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Science 20 September 1985:
Vol. 229. no. 4719, pp. 1208 - 1214
DOI: 10.1126/science.2994215
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Articles

Science, Vol 229, Issue 4719, 1208-1214
Copyright © 1985 by American Association for the Advancement of Science

articles

Genetic engineering of novel genomes of large DNA viruses

B Roizman and FJ Jenkins

Analyses of the function of specific genes and sequences of large DNA viruses such as herpesviruses and poxviruses present special problems because of the size of their genomes (120 to 250 kilobase pairs). Various methods for engineering site-specific insertions or deletions based on the use of selectable markers have been developed and applied for the elucidation of the function of specific DNA sequences, the identification of genes nonessential for virus growth in cell culture, and the expression of foreign genes. These methods should also make possible the construction of viral vectors capable of delivering genes specifying antigens for the prevention of infectious diseases in humans and animals.


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