Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Science 13 September 1985:
Vol. 229. no. 4718, pp. 1097 - 1099
DOI: 10.1126/science.3929378
Prev | Table of Contents | Next

Articles

Science, Vol 229, Issue 4718, 1097-1099
Copyright © 1985 by American Association for the Advancement of Science

articles

Immunohistochemical localization in the rat brain of the precursor for thyrotropin-releasing hormone

IM Jackson, P Wu, and RM Lechan

A rabbit antiserum to a peptide sequence present in the precursor for thyrotropin-releasing hormone (proTRH), deduced from cloned amphibian-skin complementary DNA, was raised by immunization with the synthetic decapeptide Cys-Lys-Arg-Gln-His-Pro-Gly-Lys-Arg-Cys (proTRH-SH). Immunohistochemical studies on rat brain tissue showed staining of neuronal perikarya in the parvicellular division of the paraventricular nucleus of the hypothalamus and the raphe complex of the medulla, identical to that already described for thyrotropin-releasing hormone (TRH). Immunostaining was abolished by preincubation with proTRH-SH (10(-6)M) but not TRH (10(-5)M). Both TRH precursor and TRH were located in neurons of the paraventricular nucleus. However, in contrast to the findings for TRH, no staining was observed in axon terminals of the median eminence. These results suggest that a TRH precursor analogous to that reported in frog skin is present in the rat brain and that TRH in the mammalian central nervous system is a product of ribosomal biosynthesis.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Cloning and hypothalamic distribution of the chicken thyrotropin-releasing hormone precursor cDNA.
K. Vandenborne, S. A Roelens, V. M Darras, E. R Kuhn, and S. Van der Geyten (2005)
J. Endocrinol. 186, 387-396
   Abstract »    Full Text »    PDF »
The Biology of pro-Thyrotropin-Releasing Hormone-Derived Peptides.
E. A. Nillni and K. A. Sevarino (1999)
Endocr. Rev. 20, 599-648
   Abstract »    Full Text »
The Corticotropin-Release Inhibitory Factor Hypothesis: A Review of the Evidence for the Existence of Inhibitory as Well as Stimulatory Hypophysiotropic Regulation of Adrenocorticotropin Secretion and Biosynthesis.
D. Engler, E. Redei, and I. Kola (1999)
Endocr. Rev. 20, 460-500
   Abstract »    Full Text »    PDF »
Thyroid hormone regulates TRH biosynthesis in the paraventricular nucleus of the rat hypothalamus.
T. Segerson, J Kauer, H. Wolfe, H Mobtaker, P Wu, I. Jackson, and R. Lechan (1987)
Science 238, 78-80
   Abstract »    PDF »
Thyrotropin-releasing hormone precursor: characterization in rat brain.
R. Lechan, P Wu, I. Jackson, H Wolf, S Cooperman, G Mandel, and R. Goodman (1986)
Science 231, 159-161
   Abstract »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)