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Science 12 July 1985:
Vol. 229. no. 4709, pp. 171 - 174
DOI: 10.1126/science.2990045
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Articles

Science, Vol 229, Issue 4709, 171-174
Copyright © 1985 by American Association for the Advancement of Science

articles

Discovery of a predicted DNA knot substantiates a model for site-specific recombination

SA Wasserman, JM Dungan, and NR Cozzarelli

The mechanism of site-specific genetic recombination mediated by Tn3 resolvase has been investigated by a topological approach. Extrapolation of a detailed model of synapsis and strand exchange predicts the formation of an additional DNA product with a specific knotted structure. Two-dimensional gel electrophoresis of DNA reacted in vitro revealed a product, about 0.1 percent of the total, with the appropriate mobility. A technique for determining DNA topology by electron microscopy was improved such that less than a nanogram of DNA was required. The structure of the knot was as predicted, providing strong evidence for the model and showing the power of the topological method.


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