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Science 5 July 1985:
Vol. 229. no. 4708, pp. 77 - 79
DOI: 10.1126/science.3892687
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Articles

Science, Vol 229, Issue 4708, 77-79
Copyright © 1985 by American Association for the Advancement of Science

articles

Immunocytochemical detection of acetylcholine in the rat central nervous system

M Geffard, A McRae-Degueurce, and ML Souan

A specific antibody to acetylcholine was raised and used as a marker for cholinergic neurons in the rat central nervous system. The acetylcholine conjugate was obtained by a two-step immunogen synthesis procedure. An enzyme-linked immunosorbent assay was used to test the specificity and affinity of the antibody in vitro; the results indicated high affinity. A chemical perfusion mixture of allyl alcohol and glutaraldehyde was used to fix the acetylcholine in the nervous tissue. Peroxidase-antiperoxidase immunocytochemistry showed many acetylcholine-immunoreactive cells and fibers in sections from the medial septum region.


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