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Science 5 July 1985:
Vol. 229. no. 4708, pp. 65 - 67
DOI: 10.1126/science.2990039
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Articles

Science, Vol 229, Issue 4708, 65-67
Copyright © 1985 by American Association for the Advancement of Science

articles

A role for glycosylation of the alpha subunit in transduction of biological signal in glycoprotein hormones

MR Sairam and GN Bhargavi

The biological properties of recombinants of glycoprotein hormones in which the alpha and beta subunits were differentially deglycosylated have been investigated. Specific deglycosylation of the alpha subunit generated a recombinant that had more receptor-binding activity but did not produce hormone response in the target cells. The deglycosylated alpha + beta recombinant was also an antagonist of the action of the native hormone. Thus, the carbohydrates in the alpha subunit play a dominant role in the transduction of the hormone signal into the cell.


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