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Science 28 June 1985:
Vol. 228. no. 4707, pp. 1543 - 1544
DOI: 10.1126/science.2990033
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Articles

Science, Vol 228, Issue 4707, 1543-1544
Copyright © 1985 by American Association for the Advancement of Science

articles

Imaging elemental distribution and ion transport in cultured cells with ion microscopy

S Chandra and GH Morrison

Both elemental distribution and ion transport in cultured cells have been imaged by ion microscopy. Morphological and chemical information was obtained with a spatial resolution of approximately 0.5 micron for sodium, potassium, calcium, and magnesium in freeze-fixed, cryofractured, and freeze-dried normal rat kidney cells and Chinese hamster ovary cells. Ion transport was successfully demonstrated by imaging Na+-K+ fluxes after the inhibition of Na+- and K+ -dependent adenosine triphosphatase with ouabain. This method allows measurements of elemental (isotopic) distribution to be related to cell morphology, thereby providing the means for studying ion distribution and ion transport under different physiological, pathological, and toxicological conditions in cell culture systems.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Imaging of Fluorine and Boron from Fluorinated Boronophenylalanine in the Same Cell at Organelle Resolution by Correlative Ion Microscopy and Confocal Laser Scanning Microscopy.
S. Chandra, G. W. Kabalka, D. R. Lorey II, D. R. Smith, and J. A. Coderre (2002)
Clin. Cancer Res. 8, 2675-2683
   Abstract »    Full Text »    PDF »
Fluorescence digital imaging microscopy in cell biology.
D. Arndt-Jovin, M Robert-Nicoud, S. Kaufman, and T. Jovin (1985)
Science 230, 247-256
   Abstract »    PDF »


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