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Science 14 June 1985: Vol. 228. no. 4705, pp. 1315 - 1317 DOI: 10.1126/science.4001944
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Articles
Science, Vol 228, Issue 4705, 1315-1317
Copyright © 1985 by American Association for the Advancement of Science
Filamentous fusion phage: novel expression vectors that display cloned antigens on the virion surface
GP Smith
Foreign DNA fragments can be inserted into filamentous phage gene III to create a fusion protein with the foreign sequence in the middle. The fusion protein is incorporated into the virion, which retains infectivity and displays the foreign amino acids in immunologically accessible form. These "fusion phage" can be enriched more than 1000-fold over ordinary phage by affinity for antibody directed against the foreign sequence. Fusion phage may provide a simple way of cloning a gene when an antibody against the product of that gene is available.
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- S. R. Sompuram, V. Kodela, H. Ramanathan, C. Wescott, G. Radcliffe, and S. A. Bogen (2002)
Clin. Chem.
48, 410-420
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- Isolation of Protein Subpopulations Undergoing Protein-Protein Interactions.
- T. J. Nelson, P. S. Backlund Jr., A. L. Yergey, and D. L. Alkon (2002)
Mol. Cell. Proteomics
1, 253-259
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- Profiling the Molecular Diversity of Blood Vessels.
- R. PASQUALINI and W. ARAP (2002)
Cold Spring Harb Symp Quant Biol
67, 223-226
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- Bacterial Phage Receptors, Versatile Tools for Display of Polypeptides on the Cell Surface.
- H. Etz, D. B. Minh, C. Schellack, E. Nagy, and A. Meinke (2001)
J. Bacteriol.
183, 6924-6935
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- Selection of catalytically active biotin ligase and trypsin mutants by phage display.
- C. Heinis, A. Huber, S. Demartis, J. Bertschinger, S. Melkko, L. Lozzi, P. Neri, and D. Neri (2001)
Protein Eng. Des. Sel.
14, 1043-1052
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- Inhibition of antibody-dependent stimulation of lipoteichoic acid-treated human monocytes and macrophages by polyglycerolphosphate-reactive peptides.
- A. Gargir, I. Ofek, D. Hasty, S. Meron-Sudai, H. Tsubery, Y. Keisari, and A. Nissim (2001)
J. Leukoc. Biol.
70, 537-542
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- Rapid and precise epitope mapping of monoclonal antibodies against Plasmodium falciparum AMA1 by combined phage display of fragments and random peptides.
- A. M. Coley, N. V. Campanale, J. L. Casey, A. N. Hodder, P. E. Crewther, R. F. Anders, L. M. Tilley, and M. Foley (2001)
Protein Eng. Des. Sel.
14, 691-698
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- Allergen mimotopes for 3-dimensional epitope search and induction of antibodies inhibiting human IgE.
- E. GANGLBERGER, K. GRÜNBERGER, B. SPONER, C. RADAUER, H. BREITENEDER, G. BOLTZ-NITULESCU, O. SCHEINER, and E. JENSEN-JAROLIM (2000)
FASEB J
14, 2177-2184
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