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Science 14 June 1985:
Vol. 228. no. 4705, pp. 1315 - 1317
DOI: 10.1126/science.4001944

Articles

Science, Vol 228, Issue 4705, 1315-1317
Copyright © 1985 by American Association for the Advancement of Science


articles

Filamentous fusion phage: novel expression vectors that display cloned antigens on the virion surface

GP Smith

Foreign DNA fragments can be inserted into filamentous phage gene III to create a fusion protein with the foreign sequence in the middle. The fusion protein is incorporated into the virion, which retains infectivity and displays the foreign amino acids in immunologically accessible form. These "fusion phage" can be enriched more than 1000-fold over ordinary phage by affinity for antibody directed against the foreign sequence. Fusion phage may provide a simple way of cloning a gene when an antibody against the product of that gene is available.


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Allergen mimotopes for 3-dimensional epitope search and induction of antibodies inhibiting human IgE.
E. GANGLBERGER, K. GRÜNBERGER, B. SPONER, C. RADAUER, H. BREITENEDER, G. BOLTZ-NITULESCU, O. SCHEINER, and E. JENSEN-JAROLIM (2000)
FASEB J 14, 2177-2184
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Science. ISSN 0036-8075 (print), 1095-9203 (online)